TY - GEN
T1 - On the Entropic Heating of Microfluidics for PCR Applications
AU - Walsh, E. J.
AU - Walsh, P. A.
AU - Grimes, R.
AU - Davies, D. M.R.
PY - 2003
Y1 - 2003
N2 - Polymerized Chain Reaction (PCR) technology has for a number of years been used as a tool for a range of applications in genetic research. Indeed, in the world of genetics PCR is considered to be one of the most important discoveries of the last century. This work is focused upon understanding and utilizing the thermodynamics of the fluid flow through microsystems, with the aim of developing an efficient thermocycler for PCR applications. The novel approach of utilizing the entropy generation rate in a couette type flow to provide entropic heating to the bio-fluid is addressed. The advantages of such a PCR device include, the removal of external heaters, an even distribution of heating through the bio-fluid, possibility of enhanced mixing, fast cycle times and the requirement for small sample sizes. These parameters are key requirements for an efficient PCR on the microscale. It is also demonstrated that this concept is not possible on the macro scale due to the ratio of heat generation relatively to fluid volume, however on the micro scale it is demonstrated to be theoretically plausible.
AB - Polymerized Chain Reaction (PCR) technology has for a number of years been used as a tool for a range of applications in genetic research. Indeed, in the world of genetics PCR is considered to be one of the most important discoveries of the last century. This work is focused upon understanding and utilizing the thermodynamics of the fluid flow through microsystems, with the aim of developing an efficient thermocycler for PCR applications. The novel approach of utilizing the entropy generation rate in a couette type flow to provide entropic heating to the bio-fluid is addressed. The advantages of such a PCR device include, the removal of external heaters, an even distribution of heating through the bio-fluid, possibility of enhanced mixing, fast cycle times and the requirement for small sample sizes. These parameters are key requirements for an efficient PCR on the microscale. It is also demonstrated that this concept is not possible on the macro scale due to the ratio of heat generation relatively to fluid volume, however on the micro scale it is demonstrated to be theoretically plausible.
UR - http://www.scopus.com/inward/record.url?scp=1942520370&partnerID=8YFLogxK
U2 - 10.1115/imece2003-41423
DO - 10.1115/imece2003-41423
M3 - Conference contribution
AN - SCOPUS:1942520370
SN - 0791837211
SN - 9780791837214
T3 - American Society of Mechanical Engineers, Micro-Electromechanical Systems Division Publication (MEMS)
SP - 33
EP - 36
BT - Micro-Electro-Mechanical Systems (MEMS) - 2003
PB - American Society of Mechanical Engineers
T2 - 2003 ASME International Mechanical Engineering Congress
Y2 - 15 November 2003 through 21 November 2003
ER -