Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins

Julie B. O'Brien; Matthew S. McCabe; Verónica Athié-Morales; George S.A. McDonald; Déirdre B. Ní Eidhin; Dermot P. Kelleher

doi:10.1016/j.femsle.2005.04.005

Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins

Julie B. O'Brien, Matthew S. McCabe, Verónica Athié-Morales, George S.A. McDonald, Déirdre B. Ní Eidhin, Dermot P. Kelleher

Research output: Contribution to journal › Article › peer-review

Abstract

Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.

Original language	English
Pages (from-to)	199-205
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	246
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2005.04.005
Publication status	Published - 15 May 2005
Externally published	Yes

Keywords

Clostridium difficile
Diarrhoea
Hamster model
Surface layer proteins

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10.1016/j.femsle.2005.04.005

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title = "Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins",

abstract = "Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.",

keywords = "Clostridium difficile, Diarrhoea, Hamster model, Surface layer proteins",

author = "O'Brien, {Julie B.} and McCabe, {Matthew S.} and Ver{\'o}nica Athi{\'e}-Morales and McDonald, {George S.A.} and {N{\'i} Eidhin}, {D{\'e}irdre B.} and Kelleher, {Dermot P.}",

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doi = "10.1016/j.femsle.2005.04.005",

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T1 - Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins

AU - O'Brien, Julie B.

AU - McCabe, Matthew S.

AU - Athié-Morales, Verónica

AU - McDonald, George S.A.

AU - Ní Eidhin, Déirdre B.

AU - Kelleher, Dermot P.

PY - 2005/5/15

Y1 - 2005/5/15

N2 - Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.

AB - Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.

KW - Clostridium difficile

KW - Diarrhoea

KW - Hamster model

KW - Surface layer proteins

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SN - 0378-1097

VL - 246

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins

Abstract

Keywords

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