TY - JOUR
T1 - Passive immunisation of hamsters against Clostridium difficile infection using antibodies to surface layer proteins
AU - O'Brien, Julie B.
AU - McCabe, Matthew S.
AU - Athié-Morales, Verónica
AU - McDonald, George S.A.
AU - Ní Eidhin, Déirdre B.
AU - Kelleher, Dermot P.
PY - 2005/5/15
Y1 - 2005/5/15
N2 - Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.
AB - Clostridium difficile is a major cause of antibiotic-associated diarrhoea and the primary cause of psedomembraneous colitis in hospitalised patients. We assessed the protective effect of anti-surface layer protein (SLP) antibodies on C. difficile infection in a lethal hamster challenge model. Post-challenge survival was significantly prolonged in the anti-SLP treated group compared with control groups (P = 0.0281 and P = 0.0283). The potential mechanism of action of the antiserum was shown to be through enhancement of C. difficile phagocytosis. This report indicates that anti-SLP antibodies can modulate the course of C. difficile infection and may therefore merit closer investigation for use as constituents of multi-component vaccines against C. difficile associated diarrhoea.
KW - Clostridium difficile
KW - Diarrhoea
KW - Hamster model
KW - Surface layer proteins
UR - http://www.scopus.com/inward/record.url?scp=19544374453&partnerID=8YFLogxK
U2 - 10.1016/j.femsle.2005.04.005
DO - 10.1016/j.femsle.2005.04.005
M3 - Article
C2 - 15899406
AN - SCOPUS:19544374453
SN - 0378-1097
VL - 246
SP - 199
EP - 205
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -