TY - JOUR
T1 - Perspectives for the microbial production of methyl propionate integrated with product recovery
AU - Pereira, Joana P.C.
AU - van der Wielen, Luuk A.M.
AU - Straathof, Adrie J.J.
N1 - Publisher Copyright:
© 2018 The Author(s)
PY - 2018/5
Y1 - 2018/5
N2 - A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer-Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/(L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.
AB - A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer-Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/(L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.
KW - Biomaterials
KW - Bioprocess engineering
KW - Integrated product recovery
KW - Modeling
UR - http://www.scopus.com/inward/record.url?scp=85041729507&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2018.01.118
DO - 10.1016/j.biortech.2018.01.118
M3 - Article
C2 - 29438919
AN - SCOPUS:85041729507
SN - 0960-8524
VL - 256
SP - 187
EP - 194
JO - Bioresource Technology
JF - Bioresource Technology
ER -