Photobiochemical Production of Carbon Monoxide by Thermus thermophilus ba3-Cytochrome c Oxidase

Constantinos Koutsoupakis; Tewfik Soulimane; Constantinos Varotsis

doi:10.1002/chem.201406239

Photobiochemical Production of Carbon Monoxide by Thermus thermophilus ba₃-Cytochrome c Oxidase

Constantinos Koutsoupakis, Tewfik Soulimane, Constantinos Varotsis

Department of Chemical Sciences

Cyprus University of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

We report the photobiochemical production of carbon monoxide by a terminal ba₃-cytochrome c oxidase from T. thermophilus HB8. FTIR and time-resolved step-scan FTIR spectroscopies were combined to probe this process and also monitor the concomitant binding of the produced gas to other intact ba₃ molecules forming the ba₃-CO complex. The activation of this mechanism by ba₃-oxidase under visible excitation raises the question as to whether such a mechanism is physiologically relevant to the extreme environment in which it operates.

Original language	English
Pages (from-to)	4958-4961
Number of pages	4
Journal	Chemistry - A European Journal
Volume	21
Issue number	13
DOIs	https://doi.org/10.1002/chem.201406239
Publication status	Published - 23 Mar 2015

Keywords

biochemistry
carbon monoxide
FTIR spectroscopy
heme proteins
oxidoreductases

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10.1002/chem.201406239

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title = "Photobiochemical Production of Carbon Monoxide by Thermus thermophilus ba3-Cytochrome c Oxidase",

abstract = "We report the photobiochemical production of carbon monoxide by a terminal ba3-cytochrome c oxidase from T. thermophilus HB8. FTIR and time-resolved step-scan FTIR spectroscopies were combined to probe this process and also monitor the concomitant binding of the produced gas to other intact ba3 molecules forming the ba3-CO complex. The activation of this mechanism by ba3-oxidase under visible excitation raises the question as to whether such a mechanism is physiologically relevant to the extreme environment in which it operates.",

keywords = "biochemistry, carbon monoxide, FTIR spectroscopy, heme proteins, oxidoreductases",

author = "Constantinos Koutsoupakis and Tewfik Soulimane and Constantinos Varotsis",

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AU - Koutsoupakis, Constantinos

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AU - Varotsis, Constantinos

PY - 2015/3/23

Y1 - 2015/3/23

N2 - We report the photobiochemical production of carbon monoxide by a terminal ba3-cytochrome c oxidase from T. thermophilus HB8. FTIR and time-resolved step-scan FTIR spectroscopies were combined to probe this process and also monitor the concomitant binding of the produced gas to other intact ba3 molecules forming the ba3-CO complex. The activation of this mechanism by ba3-oxidase under visible excitation raises the question as to whether such a mechanism is physiologically relevant to the extreme environment in which it operates.

AB - We report the photobiochemical production of carbon monoxide by a terminal ba3-cytochrome c oxidase from T. thermophilus HB8. FTIR and time-resolved step-scan FTIR spectroscopies were combined to probe this process and also monitor the concomitant binding of the produced gas to other intact ba3 molecules forming the ba3-CO complex. The activation of this mechanism by ba3-oxidase under visible excitation raises the question as to whether such a mechanism is physiologically relevant to the extreme environment in which it operates.

KW - biochemistry

KW - carbon monoxide

KW - FTIR spectroscopy

KW - heme proteins

KW - oxidoreductases

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JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

IS - 13

ER -

Photobiochemical Production of Carbon Monoxide by Thermus thermophilus ba₃-Cytochrome c Oxidase

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