TY - JOUR
T1 - Polyphenols of jabuticaba [Myrciaria jaboticaba (Vell.) O.Berg] seeds incorporated in a yogurt model exert antioxidant activity and modulate gut microbiota of 1,2-dimethylhydrazine-induced colon cancer in rats
AU - Fidelis, Marina
AU - Santos, Jânio Sousa
AU - Escher, Graziela Bragueto
AU - Rocha, Ramon Silva
AU - Cruz, Adriano Gomes
AU - Cruz, Thiago Mendanha
AU - Marques, Mariza Boscacci
AU - Nunes, Juliana Barbosa
AU - do Carmo, Mariana Araújo Vieira
AU - de Almeida, Leonardo Augusto
AU - Kaneshima, Tai
AU - Azevedo, Luciana
AU - Granato, Daniel
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/1/1
Y1 - 2021/1/1
N2 - The chemical composition, antioxidant activity (AA), cytotoxic activity, antihemolytic effects, and enzyme inhibition (EI) of lyophilized jabuticaba (Myrciaria jaboticaba) seed extract (LJE) was studied. The main compounds found were castalagin, vescalagin, procyanidin A2, and ellagic acid. LJE was more toxic to cancer cells than to normal cells, meaning relative toxicological safety. This cytotoxic effect can be attributed to the pro-oxidant effect observed in the reactive oxygen species (ROS) generation assay. LJE inhibited α-amylase, α-glucosidase, and ACE-I activities and protected human erythrocytes from hemolysis. LJE was incorporated into yogurts at different concentrations and the total phenolic content, AA, and EI increased in a dose-dependent manner. LJE-containing yogurt presented 86% sensory acceptance. The yogurt was administered to Wistar rats bearing cancer and it modulated the gut bacterial microbiota, having a prebiotic effect. LJE is a potential functional ingredient for food companies looking for TPC, AA, and prebiotic effect in vivo.
AB - The chemical composition, antioxidant activity (AA), cytotoxic activity, antihemolytic effects, and enzyme inhibition (EI) of lyophilized jabuticaba (Myrciaria jaboticaba) seed extract (LJE) was studied. The main compounds found were castalagin, vescalagin, procyanidin A2, and ellagic acid. LJE was more toxic to cancer cells than to normal cells, meaning relative toxicological safety. This cytotoxic effect can be attributed to the pro-oxidant effect observed in the reactive oxygen species (ROS) generation assay. LJE inhibited α-amylase, α-glucosidase, and ACE-I activities and protected human erythrocytes from hemolysis. LJE was incorporated into yogurts at different concentrations and the total phenolic content, AA, and EI increased in a dose-dependent manner. LJE-containing yogurt presented 86% sensory acceptance. The yogurt was administered to Wistar rats bearing cancer and it modulated the gut bacterial microbiota, having a prebiotic effect. LJE is a potential functional ingredient for food companies looking for TPC, AA, and prebiotic effect in vivo.
KW - Castalagin
KW - Cytotoxic activity
KW - Dairy products
KW - Fruit seeds
KW - Gut microbiota modulation
KW - Vescalagin
UR - http://www.scopus.com/inward/record.url?scp=85088376552&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2020.127565
DO - 10.1016/j.foodchem.2020.127565
M3 - Article
C2 - 32717686
AN - SCOPUS:85088376552
SN - 0308-8146
VL - 334
SP - 127565
JO - Food Chemistry
JF - Food Chemistry
M1 - 127565
ER -
