TY - JOUR
T1 - Potential bioactive effects of casein hydrolysates on human cultured cells
AU - Phelan, Martha
AU - Aherne-Bruce, S. Aisling
AU - O'Sullivan, Dara
AU - FitzGerald, Richard J.
AU - O'Brien, Nora M.
PY - 2009/5
Y1 - 2009/5
N2 - The potential bioactivity of eight distinct casein hydrolysates (designated a-h) was assessed by investigating different parameters on human cultured cells. Following 24 h supplementation, the casein hydrolysates exerted varying effects on the viability and growth of Jurkat T cells, with IC50 values ranging from 19.5% to 66.8% (v/v). Treatment with the hydrolysates did not affect the membrane integrity or superoxide dismutase activity of Jurkat cells. Sample a significantly affected both cellular catalase activity and reduced glutathione (GSH) content, whereas samples c, d, and e enhanced (P < 0.05) GSH content. Neither genotoxic nor genoprotective effects were exerted by the casein hydrolysates. Interestingly, the casein hydrolysates d-h significantly increased Concanavalin A (ConA)-stimulated IL-2 levels but had no effect on ConA-induced IL-10 production in the Jurkat cells. The differing bioactive effects of these casein hydrolysates may, in part, be attributed to differences in the enzyme specificities of the enzyme activities used in their preparation.
AB - The potential bioactivity of eight distinct casein hydrolysates (designated a-h) was assessed by investigating different parameters on human cultured cells. Following 24 h supplementation, the casein hydrolysates exerted varying effects on the viability and growth of Jurkat T cells, with IC50 values ranging from 19.5% to 66.8% (v/v). Treatment with the hydrolysates did not affect the membrane integrity or superoxide dismutase activity of Jurkat cells. Sample a significantly affected both cellular catalase activity and reduced glutathione (GSH) content, whereas samples c, d, and e enhanced (P < 0.05) GSH content. Neither genotoxic nor genoprotective effects were exerted by the casein hydrolysates. Interestingly, the casein hydrolysates d-h significantly increased Concanavalin A (ConA)-stimulated IL-2 levels but had no effect on ConA-induced IL-10 production in the Jurkat cells. The differing bioactive effects of these casein hydrolysates may, in part, be attributed to differences in the enzyme specificities of the enzyme activities used in their preparation.
UR - http://www.scopus.com/inward/record.url?scp=60349097219&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2008.12.004
DO - 10.1016/j.idairyj.2008.12.004
M3 - Article
AN - SCOPUS:60349097219
SN - 0958-6946
VL - 19
SP - 279
EP - 285
JO - International Dairy Journal
JF - International Dairy Journal
IS - 5
ER -