Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties

Alexander T. Hanke, Marieke E. Klijn, Peter D.E.M. Verhaert, Luuk A.M. van der Wielen, Marcel Ottens, Michel H.M. Eppink, Emile J.A.X. van de Sandt

Research output: Contribution to journalArticlepeer-review

Abstract

The correlation between the dimensionless retention times (DRT) of proteins in hydrophobic interaction chromatography (HIC) and their surface properties were investigated. A ternary atomic-level hydrophobicity scale was used to calculate the distribution of local average hydrophobicity across the proteins surfaces. These distributions were characterized by robust descriptive statistics to reduce their sensitivity to small changes in the three-dimensional structure. The applicability of these statistics for the prediction of protein retention behaviour was looked into. A linear combination of robust statistics describing the central tendency, heterogeneity and frequency of highly hydrophobic clusters was found to have a good predictive capability (R2=0.78), when combined a factor to account for protein size differences. The achieved error of prediction was 35% lower than for a similar model based on a description of the protein surface on an amino acid level. This indicates that a robust and mathematically simple model based on an atomic description of the protein surface can be used for the prediction of the retention behaviour of conformationally stable globular proteins with a well determined 3D structure in HIC.

Original languageEnglish
Pages (from-to)372-381
Number of pages10
JournalBiotechnology Progress
Volume32
Issue number2
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Keywords

  • Atomic-level surface description
  • Hydrophobic interaction chromatography
  • Protein surface properties
  • Retention time prediction
  • Robust statistics

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