Preparation and characterisation of a Ni2+/Co2+-cyclam modified mesoporous cellular foam for the specific immobilisation of His6-alanine racemase

Darragh Gaffney; Noreldeen H. Abdallah; Jakki C. Cooney; Fathima R. Laffir; Karim Engelmark Cassimjee; Per Berglund; Ulf Hanefeld; Edmond Magner

doi:10.1016/j.molcatb.2014.08.011

Preparation and characterisation of a Ni²⁺/Co²⁺-cyclam modified mesoporous cellular foam for the specific immobilisation of His₆-alanine racemase

Darragh Gaffney, Noreldeen H. Abdallah, Jakki C. Cooney, Fathima R. Laffir, Karim Engelmark Cassimjee, Per Berglund, Ulf Hanefeld, Edmond Magner

Research output: Contribution to journal › Article › peer-review

Abstract

Nickel and cobalt cyclam modified mesocellular foam (MCF) materials were prepared and characterised. The metal cyclam modified materials displayed reduced surface areas and pore diameters in comparison to MCF. The modified materials were used to specifically anchor a histidine tagged form of the enzyme, alanine racemase (HT-AlaR). Non-specific adsorption was predominantly hydrophobic//hydrophilic in nature and could be significantly reduced in the presence of 2% polyethylene glycol. The activity of HT-AlaR immobilised on Ni and Co-MCF was essentially the same as that of the free enzyme, demonstrating that enzymes can be specifically immobilised within the pores of mesoporous materials in a stable and catalytically active manner.

Original language	English
Pages (from-to)	154-160
Number of pages	7
Journal	Journal of Molecular Catalysis B: Enzymatic
Volume	109
DOIs	https://doi.org/10.1016/j.molcatb.2014.08.011
Publication status	Published - Nov 2014

Keywords

His-tagged alanine racemase
Mesoporous silicates
Metal cyclam

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Gaffney, D., Abdallah, N. H., Cooney, J. C., Laffir, F. R., Cassimjee, K. E., Berglund, P., Hanefeld, U., & Magner, E. (2014). Preparation and characterisation of a Ni²⁺/Co²⁺-cyclam modified mesoporous cellular foam for the specific immobilisation of His₆-alanine racemase. Journal of Molecular Catalysis B: Enzymatic, 109, 154-160. https://doi.org/10.1016/j.molcatb.2014.08.011

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title = "Preparation and characterisation of a Ni2+/Co2+-cyclam modified mesoporous cellular foam for the specific immobilisation of His6-alanine racemase",

abstract = "Nickel and cobalt cyclam modified mesocellular foam (MCF) materials were prepared and characterised. The metal cyclam modified materials displayed reduced surface areas and pore diameters in comparison to MCF. The modified materials were used to specifically anchor a histidine tagged form of the enzyme, alanine racemase (HT-AlaR). Non-specific adsorption was predominantly hydrophobic//hydrophilic in nature and could be significantly reduced in the presence of 2% polyethylene glycol. The activity of HT-AlaR immobilised on Ni and Co-MCF was essentially the same as that of the free enzyme, demonstrating that enzymes can be specifically immobilised within the pores of mesoporous materials in a stable and catalytically active manner.",

keywords = "His-tagged alanine racemase, Mesoporous silicates, Metal cyclam",

author = "Darragh Gaffney and Abdallah, {Noreldeen H.} and Cooney, {Jakki C.} and Laffir, {Fathima R.} and Cassimjee, {Karim Engelmark} and Per Berglund and Ulf Hanefeld and Edmond Magner",

year = "2014",

month = nov,

doi = "10.1016/j.molcatb.2014.08.011",

language = "English",

volume = "109",

pages = "154--160",

journal = "Journal of Molecular Catalysis B: Enzymatic",

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Gaffney, D, Abdallah, NH, Cooney, JC , Laffir, FR, Cassimjee, KE, Berglund, P, Hanefeld, U & Magner, E 2014, 'Preparation and characterisation of a Ni²⁺/Co²⁺-cyclam modified mesoporous cellular foam for the specific immobilisation of His₆-alanine racemase', Journal of Molecular Catalysis B: Enzymatic, vol. 109, pp. 154-160. https://doi.org/10.1016/j.molcatb.2014.08.011

TY - JOUR

T1 - Preparation and characterisation of a Ni2+/Co2+-cyclam modified mesoporous cellular foam for the specific immobilisation of His6-alanine racemase

AU - Gaffney, Darragh

AU - Abdallah, Noreldeen H.

AU - Cooney, Jakki C.

AU - Laffir, Fathima R.

AU - Cassimjee, Karim Engelmark

AU - Berglund, Per

AU - Hanefeld, Ulf

AU - Magner, Edmond

PY - 2014/11

Y1 - 2014/11

N2 - Nickel and cobalt cyclam modified mesocellular foam (MCF) materials were prepared and characterised. The metal cyclam modified materials displayed reduced surface areas and pore diameters in comparison to MCF. The modified materials were used to specifically anchor a histidine tagged form of the enzyme, alanine racemase (HT-AlaR). Non-specific adsorption was predominantly hydrophobic//hydrophilic in nature and could be significantly reduced in the presence of 2% polyethylene glycol. The activity of HT-AlaR immobilised on Ni and Co-MCF was essentially the same as that of the free enzyme, demonstrating that enzymes can be specifically immobilised within the pores of mesoporous materials in a stable and catalytically active manner.

AB - Nickel and cobalt cyclam modified mesocellular foam (MCF) materials were prepared and characterised. The metal cyclam modified materials displayed reduced surface areas and pore diameters in comparison to MCF. The modified materials were used to specifically anchor a histidine tagged form of the enzyme, alanine racemase (HT-AlaR). Non-specific adsorption was predominantly hydrophobic//hydrophilic in nature and could be significantly reduced in the presence of 2% polyethylene glycol. The activity of HT-AlaR immobilised on Ni and Co-MCF was essentially the same as that of the free enzyme, demonstrating that enzymes can be specifically immobilised within the pores of mesoporous materials in a stable and catalytically active manner.

KW - His-tagged alanine racemase

KW - Mesoporous silicates

KW - Metal cyclam

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U2 - 10.1016/j.molcatb.2014.08.011

DO - 10.1016/j.molcatb.2014.08.011

M3 - Article

AN - SCOPUS:84907207324

SN - 1381-1177

VL - 109

SP - 154

EP - 160

JO - Journal of Molecular Catalysis B: Enzymatic

JF - Journal of Molecular Catalysis B: Enzymatic

ER -

Preparation and characterisation of a Ni²⁺/Co²⁺-cyclam modified mesoporous cellular foam for the specific immobilisation of His₆-alanine racemase

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Keywords

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