Preparation, optimization, and structures, of cross-linked enzyme aggregates (CLEAs)

R. Schoevaart; M. W. Wolbers; M. Golubovic; M. Ottens; A. P.G. Kieboom; F. Van Rantwijk; L. A.M. Van Der Wielen; R. A. Sheldon

doi:10.1002/bit.20184

Preparation, optimization, and structures, of cross-linked enzyme aggregates (CLEAs)

R. Schoevaart, M. W. Wolbers, M. Golubovic, M. Ottens, A. P.G. Kieboom, F. Van Rantwijk, L. A.M. Van Der Wielen, R. A. Sheldon

Research output: Contribution to journal › Article › peer-review

Abstract

The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.

Original language	English
Pages (from-to)	754-762
Number of pages	9
Journal	Biotechnology and Bioengineering
Volume	87
Issue number	6
DOIs	https://doi.org/10.1002/bit.20184
Publication status	Published - 20 Sep 2004
Externally published	Yes

Keywords

CLEA
CLEC
Immobilization
Protein

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10.1002/bit.20184

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abstract = "The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.",

keywords = "CLEA, CLEC, Immobilization, Protein",

author = "R. Schoevaart and Wolbers, {M. W.} and M. Golubovic and M. Ottens and Kieboom, {A. P.G.} and {Van Rantwijk}, F. and {Van Der Wielen}, {L. A.M.} and Sheldon, {R. A.}",

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AU - Schoevaart, R.

AU - Wolbers, M. W.

AU - Golubovic, M.

AU - Ottens, M.

AU - Kieboom, A. P.G.

AU - Van Rantwijk, F.

AU - Van Der Wielen, L. A.M.

AU - Sheldon, R. A.

PY - 2004/9/20

Y1 - 2004/9/20

N2 - The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.

AB - The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.

KW - CLEA

KW - CLEC

KW - Immobilization

KW - Protein

UR - http://www.scopus.com/inward/record.url?scp=4644232857&partnerID=8YFLogxK

U2 - 10.1002/bit.20184

DO - 10.1002/bit.20184

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EP - 762

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 6

ER -

Preparation, optimization, and structures, of cross-linked enzyme aggregates (CLEAs)

Abstract

Keywords

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