Purification and characterisation of a cellulose binding endoxylanase from Cellulomonas flavigena

J. Tony Pembroke; Michael McMahon; Bernadette Sweeney

doi:10.1007/BF01190648

Purification and characterisation of a cellulose binding endoxylanase from Cellulomonas flavigena

J. Tony Pembroke, Michael McMahon, Bernadette Sweeney

Retired Staff

University of Limerick

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Abstract

An extracellular endoxylanase capable of binding to avicel was isolated and purified from Cellulomonas flavigena. The purified xylanase, posessing no cellulose hydrolysing activity, had a molecular weight of 53K as determined electrophoretically. Steady state adsorption to avicel was reached within 5 minutes at 20°C. The adsorption followed the Langmuir isotherm with a dissociation constant at 0°C of 7.9E-8M and a saturation coefficient of 12 mg per gram with binding being stable for a wide range of ionic strengths down to 0.1M.

Original language	English
Pages (from-to)	331-334
Number of pages	4
Journal	Biotechnology Letters
Volume	17
Issue number	3
DOIs	https://doi.org/10.1007/BF01190648
Publication status	Published - Mar 1995

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title = "Purification and characterisation of a cellulose binding endoxylanase from Cellulomonas flavigena",

abstract = "An extracellular endoxylanase capable of binding to avicel was isolated and purified from Cellulomonas flavigena. The purified xylanase, posessing no cellulose hydrolysing activity, had a molecular weight of 53K as determined electrophoretically. Steady state adsorption to avicel was reached within 5 minutes at 20°C. The adsorption followed the Langmuir isotherm with a dissociation constant at 0°C of 7.9E-8M and a saturation coefficient of 12 mg per gram with binding being stable for a wide range of ionic strengths down to 0.1M.",

author = "Pembroke, {J. Tony} and Michael McMahon and Bernadette Sweeney",

year = "1995",

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doi = "10.1007/BF01190648",

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T1 - Purification and characterisation of a cellulose binding endoxylanase from Cellulomonas flavigena

AU - Pembroke, J. Tony

AU - McMahon, Michael

AU - Sweeney, Bernadette

PY - 1995/3

Y1 - 1995/3

N2 - An extracellular endoxylanase capable of binding to avicel was isolated and purified from Cellulomonas flavigena. The purified xylanase, posessing no cellulose hydrolysing activity, had a molecular weight of 53K as determined electrophoretically. Steady state adsorption to avicel was reached within 5 minutes at 20°C. The adsorption followed the Langmuir isotherm with a dissociation constant at 0°C of 7.9E-8M and a saturation coefficient of 12 mg per gram with binding being stable for a wide range of ionic strengths down to 0.1M.

AB - An extracellular endoxylanase capable of binding to avicel was isolated and purified from Cellulomonas flavigena. The purified xylanase, posessing no cellulose hydrolysing activity, had a molecular weight of 53K as determined electrophoretically. Steady state adsorption to avicel was reached within 5 minutes at 20°C. The adsorption followed the Langmuir isotherm with a dissociation constant at 0°C of 7.9E-8M and a saturation coefficient of 12 mg per gram with binding being stable for a wide range of ionic strengths down to 0.1M.

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U2 - 10.1007/BF01190648

DO - 10.1007/BF01190648

M3 - Article

AN - SCOPUS:0028905198

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VL - 17

SP - 331

EP - 334

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

Purification and characterisation of a cellulose binding endoxylanase from Cellulomonas flavigena

Abstract

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