TY - JOUR
T1 - Purification and characterization of multifunctional peptides with in situ insulinotropic and antioxidative activity from a blue whiting (Micromesistius poutassou) protein hydrolysate
AU - Shekoohi, Niloofar
AU - Harnedy-Rothwell, Pádraigín
AU - Sharkey, Shaun
AU - Lafferty, Ryan
AU - Khatib, Neda
AU - O' Harte, Finbarr
AU - FitzGerald, Richard J.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/5
Y1 - 2024/5
N2 - Two blue whiting protein hydrolysates (BWPHs) with potent in situ and in vitro antidiabetic and antioxidant activities were fractionated. Potent fractions were analysed using LC-MS/MS. Peptides VPVE and IPQD exhibited potent in situ DPP-IV inhibitory activity (Caco-2 cells) with IC50 values of 88.79 ± 5.40 and 91.12 ± 19.73 µM, respectively. Significant decreases in ROS production observed (HepG2 cells) with MPKKE, GPAG and MPAH. MPAH and LPDDL significantly (p < 0.05) increased intracellular glutathione (GSH) concentration (150.22 and 155.80 %, respectively). MPAH (196.05 %), GPSQ (183.26 %), MPKKE (153.12 %), SPEELK (152.85 %), GPAG (144.66 %), and LPDDI (142.74 %) significantly (p < 0.05) increased intracellular catalase (CAT) activity in comparison to control. Significant increases (p < 0.05) in superoxide dismutase (SOD) activity were observed following treatment with IPDDL (118.69 %), SPEEIK (118.03 %), LPDDI (117.20 %) and APEEHTP (116.60 %). The results indicate that blue whiting-derived peptides may have potential applications as multifunctional health enhancing ingredients with a focus on glycaemic management and antioxidant activity.
AB - Two blue whiting protein hydrolysates (BWPHs) with potent in situ and in vitro antidiabetic and antioxidant activities were fractionated. Potent fractions were analysed using LC-MS/MS. Peptides VPVE and IPQD exhibited potent in situ DPP-IV inhibitory activity (Caco-2 cells) with IC50 values of 88.79 ± 5.40 and 91.12 ± 19.73 µM, respectively. Significant decreases in ROS production observed (HepG2 cells) with MPKKE, GPAG and MPAH. MPAH and LPDDL significantly (p < 0.05) increased intracellular glutathione (GSH) concentration (150.22 and 155.80 %, respectively). MPAH (196.05 %), GPSQ (183.26 %), MPKKE (153.12 %), SPEELK (152.85 %), GPAG (144.66 %), and LPDDI (142.74 %) significantly (p < 0.05) increased intracellular catalase (CAT) activity in comparison to control. Significant increases (p < 0.05) in superoxide dismutase (SOD) activity were observed following treatment with IPDDL (118.69 %), SPEEIK (118.03 %), LPDDI (117.20 %) and APEEHTP (116.60 %). The results indicate that blue whiting-derived peptides may have potential applications as multifunctional health enhancing ingredients with a focus on glycaemic management and antioxidant activity.
KW - Antioxidant
KW - Bioactive peptides
KW - Blue whiting
KW - Caco-2
KW - Glycaemic management
KW - HepG2
UR - http://www.scopus.com/inward/record.url?scp=85190140761&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2024.106173
DO - 10.1016/j.jff.2024.106173
M3 - Article
AN - SCOPUS:85190140761
SN - 1756-4646
VL - 116
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 106173
ER -