Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan

P. Van Hee; A. P.J. Middelberg; R. G.J.M. Van Der Lans; L. A.M. Van Der Wielen

doi:10.1016/j.jchromb.2004.01.052

Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan

P. Van Hee, A. P.J. Middelberg, R. G.J.M. Van Der Lans, L. A.M. Van Der Wielen

Research output: Contribution to journal › Conference article › peer-review

Abstract

Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip^® of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.

Original language	English
Pages (from-to)	111-119
Number of pages	9
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	807
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2004.01.052
Publication status	Published - 25 Jul 2004
Externally published	Yes
Event	12th International Conference on Biopartitioning and Purif. - Vancouver,BC, Canada Duration: 1 Jun 2003 → 1 Jun 2003

Keywords

Acetaldehyde
Cell debris
Escherichia coli
Muramic acid
Peptidoglycan
Pseudomonas putida

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10.1016/j.jchromb.2004.01.052

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title = "Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan",

abstract = "Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip{\textregistered} of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.",

keywords = "Acetaldehyde, Cell debris, Escherichia coli, Muramic acid, Peptidoglycan, Pseudomonas putida",

author = "{Van Hee}, P. and Middelberg, {A. P.J.} and {Van Der Lans}, {R. G.J.M.} and {Van Der Wielen}, {L. A.M.}",

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doi = "10.1016/j.jchromb.2004.01.052",

language = "English",

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pages = "111--119",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

number = "1",

note = "12th International Conference on Biopartitioning and Purif. ; Conference date: 01-06-2003 Through 01-06-2003",

}

Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan. / Van Hee, P.; Middelberg, A. P.J.; Van Der Lans, R. G.J.M. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 807, No. 1, 25.07.2004, p. 111-119.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan

AU - Van Hee, P.

AU - Middelberg, A. P.J.

AU - Van Der Lans, R. G.J.M.

AU - Van Der Wielen, L. A.M.

PY - 2004/7/25

Y1 - 2004/7/25

N2 - Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip® of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.

AB - Quantification of solid cell material (cell debris) is necessary for the optimisation of the efficiency of bioseparations. Cell debris can be quantified by detection of a component present in the cell wall that can act as a marker for cell debris. Membrane-associated proteins have previously been used as a marker for cell debris. This marker was quantified by SDS-PAGE with densiometry. In this paper cell debris quantification methods are presented that are faster and more accurate, i.e. membrane-associated protein quantification with the Protein 50 Labchip® of Agilent Technologies, or that make use of peptidoglycan as marker for cell debris, i.e. a spectrophotometric muramic acid assay.

KW - Acetaldehyde

KW - Cell debris

KW - Escherichia coli

KW - Muramic acid

KW - Peptidoglycan

KW - Pseudomonas putida

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U2 - 10.1016/j.jchromb.2004.01.052

DO - 10.1016/j.jchromb.2004.01.052

M3 - Conference article

C2 - 15177168

AN - SCOPUS:2642538341

SN - 1570-0232

VL - 807

SP - 111

EP - 119

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1

T2 - 12th International Conference on Biopartitioning and Purif.

Y2 - 1 June 2003 through 1 June 2003

ER -

Quantification of solid cell material by detection of membrane-associated proteins and peptidoglycan

Abstract

Keywords

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