Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Suzanne Crilly; Edmond Magner

doi:10.1039/c0cp00350f

Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Suzanne Crilly, Edmond Magner

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

Original language	English
Pages (from-to)	10093-10097
Number of pages	5
Journal	Physical Chemistry Chemical Physics
Volume	12
Issue number	34
DOIs	https://doi.org/10.1039/c0cp00350f
Publication status	Published - 14 Sep 2010

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abstract = "Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.",

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AB - Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

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VL - 12

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EP - 10097

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 34

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Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Abstract

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