Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Suzanne Crilly; Edmond Magner

doi:10.1039/c0cp00350f

Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Suzanne Crilly
, Edmond Magner

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

Original language	English
Pages (from-to)	10093-10097
Number of pages	5
Journal	Physical Chemistry Chemical Physics
Volume	12
Issue number	34
DOIs	https://doi.org/10.1039/c0cp00350f
Publication status	Published - 14 Sep 2010

Access to Document

10.1039/c0cp00350f

Cite this

@article{44a19193519d4b2bbc0010f077c025d1,

title = "Reversible conformational change of cytochrome c at a modified gold electrode in methanol",

abstract = "Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.",

author = "Suzanne Crilly and Edmond Magner",

year = "2010",

month = sep,

day = "14",

doi = "10.1039/c0cp00350f",

language = "English",

volume = "12",

pages = "10093--10097",

journal = "Physical Chemistry Chemical Physics",

issn = "1463-9076",

number = "34",

}

TY - JOUR

T1 - Reversible conformational change of cytochrome c at a modified gold electrode in methanol

AU - Crilly, Suzanne

AU - Magner, Edmond

PY - 2010/9/14

Y1 - 2010/9/14

N2 - Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

AB - Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

UR - https://www.scopus.com/pages/publications/77955896349

U2 - 10.1039/c0cp00350f

DO - 10.1039/c0cp00350f

M3 - Article

C2 - 20683519

AN - SCOPUS:77955896349

SN - 1463-9076

VL - 12

SP - 10093

EP - 10097

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 34

ER -

Reversible conformational change of cytochrome c at a modified gold electrode in methanol

Abstract

Access to Document

Other files and links

Fingerprint

Cite this