RNA-Seq Data Analysis in Galaxy

Bérénice Batut, Marius van den Beek, Maria A. Doyle, Nicola Soranzo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

A complete RNA-Seq analysis involves the use of several different tools, with substantial software and computational requirements. The Galaxy platform simplifies the execution of such bioinformatics analyses by embedding the needed tools in its web interface, while also providing reproducibility. Here, we describe how to perform a reference-based RNA-Seq analysis using Galaxy, from data upload to visualization and functional enrichment analysis of differentially expressed genes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages367-392
Number of pages26
DOIs
Publication statusPublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2284
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Differential gene expression
  • Functional enrichment
  • Galaxy
  • Quality control
  • Sequence mapping
  • Visualizations
  • Workflow

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