TY - JOUR
T1 - Selective recovery of polyhydroxyalkanoate inclusion bodies from fermentation broth by dissolved-air flotation
AU - van Hee, Pim
AU - Elumbaring, Andrea C.M.R.
AU - van der Lans, Rob G.J.M.
AU - Van der Wielen, Luuk A.M.
PY - 2006/5/15
Y1 - 2006/5/15
N2 - Selective dissolved-air flotation for the separation of medium-chain-length polyhydroxyalkanoate (PHA) inclusion bodies (IBs) from Pseudomonas putida cell debris is investigated. Measurements show that both P. putida cell debris and PHA IBs have an iso-electric point of approximately pH 3.5. Selective aggregation and as a result selective flotation of PHA IBs was observed near this pH. Qualitative prediction of the aggregation behaviour was possible on the basis of the Van der Waals, hydrophobic and electrostatic interactions. In some cases however, the stability of the suspension could not be explained with these forces alone. It was therefore suggested that additional interactions, such as steric/brush effects, play an important role in the aggregation process.
AB - Selective dissolved-air flotation for the separation of medium-chain-length polyhydroxyalkanoate (PHA) inclusion bodies (IBs) from Pseudomonas putida cell debris is investigated. Measurements show that both P. putida cell debris and PHA IBs have an iso-electric point of approximately pH 3.5. Selective aggregation and as a result selective flotation of PHA IBs was observed near this pH. Qualitative prediction of the aggregation behaviour was possible on the basis of the Van der Waals, hydrophobic and electrostatic interactions. In some cases however, the stability of the suspension could not be explained with these forces alone. It was therefore suggested that additional interactions, such as steric/brush effects, play an important role in the aggregation process.
KW - Cell debris
KW - Dissolved-air flotation
KW - Inclusion body
KW - Particle-particle separation
KW - Polyhydroxyalkanoate (PHA)
KW - Pseudomonas putida
KW - Recovery
UR - http://www.scopus.com/inward/record.url?scp=33748270693&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2005.11.019
DO - 10.1016/j.jcis.2005.11.019
M3 - Article
C2 - 16337647
AN - SCOPUS:33748270693
SN - 0021-9797
VL - 297
SP - 595
EP - 606
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
IS - 2
ER -