TY - JOUR
T1 - Short-term dopaminergic regulation of GABA release in dopamine deafferented caudate-putamen is not directly associated with glutamic acid decarboxylase gene expression
AU - O'Connor, William T.
AU - Lindefors, Nils
AU - Brené, Stefan
AU - Herrera-Marschitz, Mario
AU - Persson, Håkan
AU - Ungerstedt, Urban
PY - 1991/7/8
Y1 - 1991/7/8
N2 - In vivo microdialysis and in situ hybridization were combined to study dopaminergic regulation of γ-amino butyric acid (GABA) neurons in rat caudate-putamen (CPu). Potassium-stimulated GABA release in CPu was elevated following a dopamine deafferentation. Local perfusion with exogenous dopamine (50 μM) for 3h via the microdialysis probe attenuated the potassium-stimulated increase in extracellular GABA in CPu. Expression of glutamic acid decarboxylase (GAD) mRNA was also increased in the dopamine deafferented CPu. However, local perfusion with dopamine had no significant attenuating effect on the increased GAD mRNA expression. These findings indicate that dopaminergic regulation of GABA neurons in the dopamine deafferented CPu includes both a short-term effect at the level of GABA release independent of changes in GAD mRNA expression and a long-term modulation at the level of GAD gene expression.
AB - In vivo microdialysis and in situ hybridization were combined to study dopaminergic regulation of γ-amino butyric acid (GABA) neurons in rat caudate-putamen (CPu). Potassium-stimulated GABA release in CPu was elevated following a dopamine deafferentation. Local perfusion with exogenous dopamine (50 μM) for 3h via the microdialysis probe attenuated the potassium-stimulated increase in extracellular GABA in CPu. Expression of glutamic acid decarboxylase (GAD) mRNA was also increased in the dopamine deafferented CPu. However, local perfusion with dopamine had no significant attenuating effect on the increased GAD mRNA expression. These findings indicate that dopaminergic regulation of GABA neurons in the dopamine deafferented CPu includes both a short-term effect at the level of GABA release independent of changes in GAD mRNA expression and a long-term modulation at the level of GAD gene expression.
KW - Caudate-putamen
KW - Dopamine
KW - GABA
KW - Glutamic acid decarboxylase
KW - In situ hybridization
KW - Microdialysis
UR - http://www.scopus.com/inward/record.url?scp=0025875447&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(91)90761-H
DO - 10.1016/0304-3940(91)90761-H
M3 - Article
C2 - 1922949
AN - SCOPUS:0025875447
SN - 0304-3940
VL - 128
SP - 66
EP - 70
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -