Short-term dopaminergic regulation of GABA release in dopamine deafferented caudate-putamen is not directly associated with glutamic acid decarboxylase gene expression

William T. O'Connor, Nils Lindefors, Stefan Brené, Mario Herrera-Marschitz, Håkan Persson, Urban Ungerstedt

Research output: Contribution to journalArticlepeer-review

Abstract

In vivo microdialysis and in situ hybridization were combined to study dopaminergic regulation of γ-amino butyric acid (GABA) neurons in rat caudate-putamen (CPu). Potassium-stimulated GABA release in CPu was elevated following a dopamine deafferentation. Local perfusion with exogenous dopamine (50 μM) for 3h via the microdialysis probe attenuated the potassium-stimulated increase in extracellular GABA in CPu. Expression of glutamic acid decarboxylase (GAD) mRNA was also increased in the dopamine deafferented CPu. However, local perfusion with dopamine had no significant attenuating effect on the increased GAD mRNA expression. These findings indicate that dopaminergic regulation of GABA neurons in the dopamine deafferented CPu includes both a short-term effect at the level of GABA release independent of changes in GAD mRNA expression and a long-term modulation at the level of GAD gene expression.

Original languageEnglish
Pages (from-to)66-70
Number of pages5
JournalNeuroscience Letters
Volume128
Issue number1
DOIs
Publication statusPublished - 8 Jul 1991
Externally publishedYes

Keywords

  • Caudate-putamen
  • Dopamine
  • GABA
  • Glutamic acid decarboxylase
  • In situ hybridization
  • Microdialysis

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