Should we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-κB using phenolic compounds from grape by-products

Adriano Costa de Camargo, Aline Camarão Telles Biasoto, Andrés R. Schwember, Daniel Granato, Gabriela Boscariol Rasera, Marcelo Franchin, Pedro L. Rosalen, Severino Matias Alencar, Fereidoon Shahidi

Research output: Contribution to journalArticlepeer-review

Abstract

Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-κB using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-κB. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-κB (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalFood Chemistry
Volume290
DOIs
Publication statusPublished - 30 Aug 2019
Externally publishedYes

Keywords

  • Bioactive phenolics
  • Cytotoxicity
  • Ferric reducing antioxidant power
  • Folin-Ciocalteu
  • High-performance liquid chromatography
  • NF-κB activation
  • Oxygen radical absorbance capacity
  • RAW 264.7 macrophages

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