Specific PCR to identify the heavy-metal-resistant bacterium Cupriavidus metallidurans

Michael P. Ryan; Catherine C. Adley

doi:10.1007/s10295-011-1011-y

Specific PCR to identify the heavy-metal-resistant bacterium Cupriavidus metallidurans

Michael P. Ryan, Catherine C. Adley

Retired Staff

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study is to develop a polymerase chain reaction (PCR) assay for rapid detection of Cupriavidus metallidurans. PCR primers targeting the Signal transduction histidine kinase gene were designed and designated Cm-F1/Cm-R1. Strains of C. metallidurans were positively identified. The size of the PCR products was 437 bp, as expected. This PCR method enables monitoring of industrial, environmental and clinical sources for presence of C. metallidurans.

Original language	English
Pages (from-to)	1613-1615
Number of pages	3
Journal	Journal of Industrial Microbiology and Biotechnology
Volume	38
Issue number	9
DOIs	https://doi.org/10.1007/s10295-011-1011-y
Publication status	Published - Sep 2011

Keywords

Cupriavidus metallidurans
PCR primers

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10.1007/s10295-011-1011-y

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abstract = "The aim of this study is to develop a polymerase chain reaction (PCR) assay for rapid detection of Cupriavidus metallidurans. PCR primers targeting the Signal transduction histidine kinase gene were designed and designated Cm-F1/Cm-R1. Strains of C. metallidurans were positively identified. The size of the PCR products was 437 bp, as expected. This PCR method enables monitoring of industrial, environmental and clinical sources for presence of C. metallidurans.",

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AB - The aim of this study is to develop a polymerase chain reaction (PCR) assay for rapid detection of Cupriavidus metallidurans. PCR primers targeting the Signal transduction histidine kinase gene were designed and designated Cm-F1/Cm-R1. Strains of C. metallidurans were positively identified. The size of the PCR products was 437 bp, as expected. This PCR method enables monitoring of industrial, environmental and clinical sources for presence of C. metallidurans.

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KW - PCR primers

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ER -

Specific PCR to identify the heavy-metal-resistant bacterium Cupriavidus metallidurans

Abstract

Keywords

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