Spectroscopic and kinetic investigation of the fully reduced and mixed valence states of ba3-cytochrome c oxidase from thermus thermophilus a fourier transform infrared (FTIR) and time-resolved step-scan FTIR study

Constantinos Koutsoupakis, Tewfik Soulimane, Constantinos Varotsis

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cytochrome c oxidase reduces O2 to H2O, a reaction coupled to proton translocation across the membrane. Results: Photolysis of CO from the mixed valence form of cytochrome ba3-CO does not lead to a heme a33+-CuB 1+-CO binuclear center. Conclusion: The absence of reverse electron transfer between hemes a3 and b is shown. Significance: Unique thermodynamic and kinetic properties of cytochrome ba3 oxidase are presented.

Original languageEnglish
Pages (from-to)37495-37507
Number of pages13
JournalJournal of Biological Chemistry
Volume287
Issue number44
DOIs
Publication statusPublished - 26 Oct 2012

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