Stability and bioactivity of a Bowman-Birk inhibitor in orange juice during processing and storage

Miryam Amigo-Benavent, Chiara Nitride, Laura Bravo, Pasquale Ferranti, M. Dolores Del Castillo

Research output: Contribution to journalArticlepeer-review

Abstract

The present research aimed to evaluate the feasibility of a soybean Bowman-Birk inhibitor (BBI) as a natural functional food ingredient. The influence of food processing, storage and digestion on the structure and bioactivity of BBI added to a food matrix was evaluated. The cytotoxic effect of the digested samples was also tested with the aim to provide information regarding the safe use of BBI as a functional food ingredient. Samples of freshly squeezed orange juice (OJ) and two orange juice model systems (OJ-ms) supplemented and non-supplemented with BBI were prepared and processed mimicking pasteurization and sterilization industrial conditions. Moreover, pasteurized samples were stored at 4 °C for two months. The samples were digested in vitro under oral-gastrointestinal physiological conditions. Results seem to indicate that the activity of BBI supplemented to the food matrix sufficiently survives thermal processing, storage and digestion processes. Changes in BBI bioactivity may be ascribed among others to the Maillard reaction. Formation of early Maillard reaction products also called Amadori products has been detected in the food sample. No cytotoxic effect was observed for the samples under study. In conclusion, our findings support that BBI has significant potential as a natural functional food ingredient in pasteurized orange juice stored at 4 °C.

Original languageEnglish
Pages (from-to)1051-1060
Number of pages10
JournalFood and Function
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

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