Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials

L. M. Davis; A. Callanan; A. V. Piterina; B. J. Doyle; M. T. Walsh; T. M. McGloughlin

doi:10.1007/978-3-642-14515-5_36

Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials

L. M. Davis
, A. Callanan
, A. V. Piterina
, B. J. Doyle
, M. T. Walsh
, T. M. McGloughlin

University of Limerick

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Effective arterial replacements for the treatment of cardiovascular diseases is still an unaddressed worldwide problem. Naturally derived biological scaffolds have huge potential in vascular treatments and have been successfully used in a variety of applications. One of the major problems with using biological scaffolds is storage, which can result in biomechanical changes in the scaffolds. This study evaluated the influence of storage of two scaffolds, small intestinal submucosa (SIS) and urinary bladder matrix (UBM). Mechanical performance was evaluated and cellular viability and proliferation was assessed at the end of the storage period. Uniaxial tensile testing allowed the behavior of the material to be assessed and also generated ultimate tensile strength (UTS) data. Cellular performance was examined by seeding human aortic endothelial cells to the scaffold and then determining cellular proliferation rates. Based on the findings from this study, ECM materials such as SIS and UBM may have potential in cardiovascular applications using off-the-shelf storage methods prior to device delivery and implantation.

Original language	English
Title of host publication	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages	139-142
Number of pages	4
DOIs	https://doi.org/10.1007/978-3-642-14515-5_36
Publication status	Published - 2010
Event	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore Duration: 1 Aug 2010 → 6 Aug 2010

Publication series

Name	IFMBE Proceedings
Volume	31 IFMBE
ISSN (Print)	1680-0737

Conference

Conference	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Country/Territory	Singapore
City	Singapore
Period	1/08/10 → 6/08/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

extracellular matrix
mechanical properties
scaffolds
storage
tissue engineering

Access to Document

10.1007/978-3-642-14515-5_36

Cite this

Davis, L. M., Callanan, A., Piterina, A. V., Doyle, B. J., Walsh, M. T., & McGloughlin, T. M. (2010). Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (pp. 139-142). (IFMBE Proceedings; Vol. 31 IFMBE). https://doi.org/10.1007/978-3-642-14515-5_36

Davis, L. M. ; Callanan, A. ; Piterina, A. V. et al. / Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials. 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. 2010. pp. 139-142 (IFMBE Proceedings).

@inproceedings{b00aec2a30f54c93b7a2ff78d7436e1d,

title = "Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials",

abstract = "Effective arterial replacements for the treatment of cardiovascular diseases is still an unaddressed worldwide problem. Naturally derived biological scaffolds have huge potential in vascular treatments and have been successfully used in a variety of applications. One of the major problems with using biological scaffolds is storage, which can result in biomechanical changes in the scaffolds. This study evaluated the influence of storage of two scaffolds, small intestinal submucosa (SIS) and urinary bladder matrix (UBM). Mechanical performance was evaluated and cellular viability and proliferation was assessed at the end of the storage period. Uniaxial tensile testing allowed the behavior of the material to be assessed and also generated ultimate tensile strength (UTS) data. Cellular performance was examined by seeding human aortic endothelial cells to the scaffold and then determining cellular proliferation rates. Based on the findings from this study, ECM materials such as SIS and UBM may have potential in cardiovascular applications using off-the-shelf storage methods prior to device delivery and implantation.",

keywords = "extracellular matrix, mechanical properties, scaffolds, storage, tissue engineering",

author = "Davis, \{L. M.\} and A. Callanan and Piterina, \{A. V.\} and Doyle, \{B. J.\} and Walsh, \{M. T.\} and McGloughlin, \{T. M.\}",

year = "2010",

doi = "10.1007/978-3-642-14515-5\_36",

language = "English",

isbn = "9783540790389",

series = "IFMBE Proceedings",

pages = "139--142",

booktitle = "6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech",

note = "6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech ; Conference date: 01-08-2010 Through 06-08-2010",

}

Davis, LM, Callanan, A, Piterina, AV, Doyle, BJ, Walsh, MT & McGloughlin, TM 2010, Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials. in 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. IFMBE Proceedings, vol. 31 IFMBE, pp. 139-142, 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, Singapore, Singapore, 1/08/10. https://doi.org/10.1007/978-3-642-14515-5_36

Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials. / Davis, L. M.; Callanan, A.; Piterina, A. V. et al.
6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. 2010. p. 139-142 (IFMBE Proceedings; Vol. 31 IFMBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials

AU - Davis, L. M.

AU - Callanan, A.

AU - Piterina, A. V.

AU - Doyle, B. J.

AU - Walsh, M. T.

AU - McGloughlin, T. M.

PY - 2010

Y1 - 2010

N2 - Effective arterial replacements for the treatment of cardiovascular diseases is still an unaddressed worldwide problem. Naturally derived biological scaffolds have huge potential in vascular treatments and have been successfully used in a variety of applications. One of the major problems with using biological scaffolds is storage, which can result in biomechanical changes in the scaffolds. This study evaluated the influence of storage of two scaffolds, small intestinal submucosa (SIS) and urinary bladder matrix (UBM). Mechanical performance was evaluated and cellular viability and proliferation was assessed at the end of the storage period. Uniaxial tensile testing allowed the behavior of the material to be assessed and also generated ultimate tensile strength (UTS) data. Cellular performance was examined by seeding human aortic endothelial cells to the scaffold and then determining cellular proliferation rates. Based on the findings from this study, ECM materials such as SIS and UBM may have potential in cardiovascular applications using off-the-shelf storage methods prior to device delivery and implantation.

AB - Effective arterial replacements for the treatment of cardiovascular diseases is still an unaddressed worldwide problem. Naturally derived biological scaffolds have huge potential in vascular treatments and have been successfully used in a variety of applications. One of the major problems with using biological scaffolds is storage, which can result in biomechanical changes in the scaffolds. This study evaluated the influence of storage of two scaffolds, small intestinal submucosa (SIS) and urinary bladder matrix (UBM). Mechanical performance was evaluated and cellular viability and proliferation was assessed at the end of the storage period. Uniaxial tensile testing allowed the behavior of the material to be assessed and also generated ultimate tensile strength (UTS) data. Cellular performance was examined by seeding human aortic endothelial cells to the scaffold and then determining cellular proliferation rates. Based on the findings from this study, ECM materials such as SIS and UBM may have potential in cardiovascular applications using off-the-shelf storage methods prior to device delivery and implantation.

KW - extracellular matrix

KW - mechanical properties

KW - scaffolds

KW - storage

KW - tissue engineering

UR - https://www.scopus.com/pages/publications/77958018210

U2 - 10.1007/978-3-642-14515-5_36

DO - 10.1007/978-3-642-14515-5_36

M3 - Conference contribution

AN - SCOPUS:77958018210

SN - 9783540790389

T3 - IFMBE Proceedings

SP - 139

EP - 142

BT - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech

T2 - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech

Y2 - 1 August 2010 through 6 August 2010

ER -

Davis LM, Callanan A, Piterina AV, Doyle BJ, Walsh MT , McGloughlin TM. Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. 2010. p. 139-142. (IFMBE Proceedings). doi: 10.1007/978-3-642-14515-5_36

Storage effects on the mechanical and cellular performance of naturally derived extracellular matrix materials

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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