TY - GEN
T1 - Structural and functional prediction of the hypothetical protein PA2481 in Pseudomonas aeruginosa Pao1
AU - Díaz, David Alberto
AU - Barreto, George Emilio
AU - Santos, Janneth González
PY - 2014
Y1 - 2014
N2 - Pseudomonas aeruginosa is a bacterium resistant to a large number of antibiotics and disinfectants. Since the antigens are responsible for producing this resistance, and most of these are proteins, the objective of this work was to predict by computational means the 3D structure and function of PA2481 protein, determining whether is involved in the antibiotic resistance of this bacterium. In order to do this, the primary structure was analyzed computationally by using servers PROSITE, PFAM, BLAST, PROTPARAM, GLOBPLOT, and PROTSCALE. The secondary structure was obtained by the consensus of algorithms SOPM, PREDATOR, DPM, DSC, and GOR4. The 3D structure was predicted with the I-TASSER server and its stereochemical conformation was evaluated with the STRUCTURE ASSESSMENT tool. The final model was visualized with PyMol program. As a result, the 3D structure of PA2481 is proposed according to its stereochemical conformation; two domains are identified as cytochrome c. The function of the protein may be related to electron transport and proton pumping to generate ATP in Pseudomonas aeruginosa. These results allow a better understanding of the role this protein plays in the physiology of this bacterium.
AB - Pseudomonas aeruginosa is a bacterium resistant to a large number of antibiotics and disinfectants. Since the antigens are responsible for producing this resistance, and most of these are proteins, the objective of this work was to predict by computational means the 3D structure and function of PA2481 protein, determining whether is involved in the antibiotic resistance of this bacterium. In order to do this, the primary structure was analyzed computationally by using servers PROSITE, PFAM, BLAST, PROTPARAM, GLOBPLOT, and PROTSCALE. The secondary structure was obtained by the consensus of algorithms SOPM, PREDATOR, DPM, DSC, and GOR4. The 3D structure was predicted with the I-TASSER server and its stereochemical conformation was evaluated with the STRUCTURE ASSESSMENT tool. The final model was visualized with PyMol program. As a result, the 3D structure of PA2481 is proposed according to its stereochemical conformation; two domains are identified as cytochrome c. The function of the protein may be related to electron transport and proton pumping to generate ATP in Pseudomonas aeruginosa. These results allow a better understanding of the role this protein plays in the physiology of this bacterium.
KW - Cystic fibrosis
KW - External otitis
KW - Hypothetical protein
KW - PA2481
KW - Pseudomonas aeruginosa
KW - Structural modeling
UR - http://www.scopus.com/inward/record.url?scp=84894832698&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-01568-2_7
DO - 10.1007/978-3-319-01568-2_7
M3 - Conference contribution
AN - SCOPUS:84894832698
SN - 9783319015675
T3 - Advances in Intelligent Systems and Computing
SP - 47
EP - 55
BT - Advances in Computational Biology - Proceedings of the 2nd Colombian Congress on Computational Biology and Bioinformatics CCBCOL 2013
PB - Springer Verlag
T2 - 2nd Colombian Congress on Computational Biology and Bioinformatics, CCBCOL 2013
Y2 - 25 September 2013 through 27 September 2013
ER -
