TY - JOUR
T1 - Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase
AU - Djeghader, Ahmed
AU - Rossotti, Melanie
AU - Abdulkarim, Saleh
AU - Biaso, Frédéric
AU - Gerbaud, Guillaume
AU - Nitschke, Wolfgang
AU - Schoepp-Cothenet, Barbara
AU - Soulimane, Tewfik
AU - Grimaldi, Stéphane
N1 - Publisher Copyright:
© 2020 The Royal Society of Chemistry.
PY - 2020/9/4
Y1 - 2020/9/4
N2 - By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase. This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases.
AB - By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase. This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases.
UR - http://www.scopus.com/inward/record.url?scp=85090068548&partnerID=8YFLogxK
U2 - 10.1039/d0cc03634j
DO - 10.1039/d0cc03634j
M3 - Article
C2 - 32716419
AN - SCOPUS:85090068548
SN - 1359-7345
VL - 56
SP - 9850
EP - 9853
JO - Chemical Communications
JF - Chemical Communications
IS - 68
ER -