Subcellular localization of NR4A2 orphan nuclear receptor expression in human and mouse synovial joint tissue

Aisling Smyth, Martina Gogarty, Daniel Crean, Evelyn P. Murphy

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

NR4A1-3 receptors are required in inflammatory disease initiation and progression, where they function as early response regulators, controlling the extent of the inflammatory response and promoting inflammatory resolution. NR4A receptor activity controls inflammatory processes in several diseases characterized by chronic inflammation including rheumatoid arthritis (RA) and atherosclerosis. Studies indicate that cell-type and cellular microenvironment can alter NR4A1-3 receptor activity and influence their biological roles. Thus, the study of appropriate in vivo models of inflammatory disease is important to ascertain their cell- and tissue-specific functional roles. Here we describe immunohistochemical approaches optimized to study the expression patterns of NR4A nuclear receptors in inflamed synovium tissues obtained from patients diagnosed with RA and mouse models of inflammatory joint disease.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages17-26
Number of pages10
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1966
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Immunofluorescence
  • Immunohistochemical analysis
  • NR4A nuclear receptors
  • Rheumatoid arthritis
  • Synovial joint tissue
  • TNF mouse model

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