TY - JOUR
T1 - Substrate inhibition and product degradation during the reduction of 4-oxoisophorone by Saccharomyces cerevisiae
AU - Buque-Taboada, Evelyn M.
AU - Straathof, Adrie J.J.
AU - Heijnen, Joseph J.
AU - Van Der Wielen, Luuk A.M.
PY - 2005/11/1
Y1 - 2005/11/1
N2 - The aromatic diketone 6R-dihydro-oxoisophorone (DOIP) is an important intermediate in the synthesis of some naturally occurring carotenoids. Its preparation via the reduction of 4-oxoisophorone (OIP) by baker's yeast has previously been developed to a pilot-scale process. In this work, the kinetics of substrate inhibition and of product degradation during the reduction of OIP using resting baker's yeast cells as catalyst is studied. Substrate inhibition during the reduction can be described by a non-competitive type of inhibition. Product is degraded to an unwanted by-product 4S,6R-actinol by baker's yeast. This reaction can very well be described by a second-order rate equation with respect to DOIP concentration, which is an exceptional case for a whole-cell-catalyzed reaction system.
AB - The aromatic diketone 6R-dihydro-oxoisophorone (DOIP) is an important intermediate in the synthesis of some naturally occurring carotenoids. Its preparation via the reduction of 4-oxoisophorone (OIP) by baker's yeast has previously been developed to a pilot-scale process. In this work, the kinetics of substrate inhibition and of product degradation during the reduction of OIP using resting baker's yeast cells as catalyst is studied. Substrate inhibition during the reduction can be described by a non-competitive type of inhibition. Product is degraded to an unwanted by-product 4S,6R-actinol by baker's yeast. This reaction can very well be described by a second-order rate equation with respect to DOIP concentration, which is an exceptional case for a whole-cell-catalyzed reaction system.
KW - 4-Oxoisophorone
KW - Baker's yeast
KW - Inhibition
KW - Kinetics
KW - Levodione
KW - Reduction
UR - http://www.scopus.com/inward/record.url?scp=24944503111&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2005.04.019
DO - 10.1016/j.enzmictec.2005.04.019
M3 - Article
AN - SCOPUS:24944503111
SN - 0141-0229
VL - 37
SP - 625
EP - 633
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 6
ER -