Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil

Anna Koprivova, Achim Schmalenberger, Stanislav Kopriva

Research output: Contribution to journalArticlepeer-review

Abstract

Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew the plants in soil/sand mix under control conditions and measured the sulfatase activity in soil using a spectrophotometric determination of the product. This protocol can be used to test the contribution of individual genes to control of microbiome assembly through analysis of mutants as well as the influence of environment on plant-microbe interactions.

Original languageEnglish
Article number1232
Pages (from-to)e3490
JournalBio-protocol
Volume10
Issue number2
DOIs
Publication statusPublished - 20 Jan 2020

Keywords

  • Arabidopsis
  • Bacteria
  • Microbiome
  • Organic sulfur
  • Plant-microbe interactions
  • Soil
  • Sulfatase

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