TY - JOUR
T1 - Surface charge and carbon contamination on an electron-beam-irradiated hydroxyapatite thin film investigated by photoluminescence and phase imaging in atomic force microscopy
AU - Hristu, Radu
AU - Tranca, Denis E.
AU - Stanciu, Stefan G.
AU - Gregor, Maros
AU - Plecenik, Tomas
AU - Truchly, Martin
AU - Roch, Tomas
AU - Tofail, Syed A.M.
AU - Stanciu, George A.
PY - 2014/4
Y1 - 2014/4
N2 - The surface properties of hydroxyapatite, including electric charge, can influence the biological response, tissue compatibility, and adhesion of biological cells and biomolecules. Results reported here help in understanding this influence by creating charged domains on hydroxyapatite thin films deposited on silicon using electron beam irradiation and investigating their shape, properties, and carbon contamination for different doses of incident injected charge by two methods. Photoluminescence laser scanning microscopy was used to image electrostatic charge trapped at pre-existing and irradiation-induced defects within these domains, while phase imaging in atomic force microscopy was used to image the carbon contamination. Scanning Auger electron spectroscopy and Kelvin probe force microscopy were used as a reference for the atomic force microscopy phase contrast and photoluminescence laser scanning microscopy measurements. Our experiment shows that by combining the two imaging techniques the effects of trapped charge and carbon contamination can be separated. Such separation yields new possibilities for advancing the current understanding of how surface charge influences mediation of cellular and protein interactions in biomaterials.
AB - The surface properties of hydroxyapatite, including electric charge, can influence the biological response, tissue compatibility, and adhesion of biological cells and biomolecules. Results reported here help in understanding this influence by creating charged domains on hydroxyapatite thin films deposited on silicon using electron beam irradiation and investigating their shape, properties, and carbon contamination for different doses of incident injected charge by two methods. Photoluminescence laser scanning microscopy was used to image electrostatic charge trapped at pre-existing and irradiation-induced defects within these domains, while phase imaging in atomic force microscopy was used to image the carbon contamination. Scanning Auger electron spectroscopy and Kelvin probe force microscopy were used as a reference for the atomic force microscopy phase contrast and photoluminescence laser scanning microscopy measurements. Our experiment shows that by combining the two imaging techniques the effects of trapped charge and carbon contamination can be separated. Such separation yields new possibilities for advancing the current understanding of how surface charge influences mediation of cellular and protein interactions in biomaterials.
KW - atomic force microscopy
KW - carbon contamination
KW - hydroxyapatite
KW - laser scanning microscopy
KW - photoluminescence
KW - surface charge
UR - http://www.scopus.com/inward/record.url?scp=84899475209&partnerID=8YFLogxK
U2 - 10.1017/S1431927614000191
DO - 10.1017/S1431927614000191
M3 - Article
C2 - 24717172
AN - SCOPUS:84899475209
SN - 1431-9276
VL - 20
SP - 586
EP - 595
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
IS - 2
ER -