Surfactant-aided size exclusion chromatography

D. A. Horneman; M. Wolbers; M. Zomerdijk; M. Ottens; J. T.F. Keurentjes; L. A.M. Van Der Wielen

doi:10.1016/j.jchromb.2004.01.061

Surfactant-aided size exclusion chromatography

D. A. Horneman, M. Wolbers, M. Zomerdijk, M. Ottens, J. T.F. Keurentjes, L. A.M. Van Der Wielen

Research output: Contribution to journal › Conference article › peer-review

Abstract

The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	807
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2004.01.061
Publication status	Published - 25 Jul 2004
Externally published	Yes
Event	12th International Conference on Biopartitioning and Purif. - Vancouver,BC, Canada Duration: 1 Jun 2003 → 1 Jun 2003

Keywords

Bovine serum albumin
Micelle-forming surfactants
Myoglobin

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10.1016/j.jchromb.2004.01.061

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title = "Surfactant-aided size exclusion chromatography",

abstract = "The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.",

keywords = "Bovine serum albumin, Micelle-forming surfactants, Myoglobin",

author = "Horneman, {D. A.} and M. Wolbers and M. Zomerdijk and M. Ottens and Keurentjes, {J. T.F.} and {Van Der Wielen}, {L. A.M.}",

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TY - JOUR

T1 - Surfactant-aided size exclusion chromatography

AU - Horneman, D. A.

AU - Wolbers, M.

AU - Zomerdijk, M.

AU - Ottens, M.

AU - Keurentjes, J. T.F.

AU - Van Der Wielen, L. A.M.

PY - 2004/7/25

Y1 - 2004/7/25

N2 - The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.

AB - The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.

KW - Bovine serum albumin

KW - Micelle-forming surfactants

KW - Myoglobin

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DO - 10.1016/j.jchromb.2004.01.061

M3 - Conference article

C2 - 15177158

AN - SCOPUS:2642529297

SN - 1570-0232

VL - 807

SP - 39

EP - 45

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1

T2 - 12th International Conference on Biopartitioning and Purif.

Y2 - 1 June 2003 through 1 June 2003

ER -

Surfactant-aided size exclusion chromatography

Abstract

Keywords

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