The growth, physiology and toxigenic potential of Bacillus cereus in cooked rice during storage temperature abuse

Ultan P. Cronin; Martin G. Wilkinson

doi:10.1016/j.foodcont.2008.10.018

The growth, physiology and toxigenic potential of Bacillus cereus in cooked rice during storage temperature abuse

Ultan P. Cronin, Martin G. Wilkinson

Department of Biological Sciences

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of storage temperature abuse on the growth and physiology of Bacillus cereus in cooked rice was examined using plate counting and flow cytometry (FCM). Concurrently, toxigenic potential was measured through recording phosphatidylcholine-specific phospholipase C (PC-PLC) activity. Rice spiked with endospores was incubated at 4 °C, 10 °C or 18 °C for 6 days. Growth was not recorded at 4 °C, whereas >1.0 × 10⁶ CFU g^-1 were detected at 10 °C and 18 °C. PC-PLC activity was temperature dependent and was not destroyed by microwaving. FCM population profiling complemented plate count data, providing novel physiological data e.g. on the membrane integrity, redox or intracellular activities of cells over time during low temperature incubation.

Original language	English
Pages (from-to)	822-828
Number of pages	7
Journal	Food Control
Volume	20
Issue number	9
DOIs	https://doi.org/10.1016/j.foodcont.2008.10.018
Publication status	Published - Sep 2009

Keywords

Bacillus cereus
Flow cytometry
Phosphatidylcholine-specific phospholipase C
Rice

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10.1016/j.foodcont.2008.10.018

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abstract = "The effect of storage temperature abuse on the growth and physiology of Bacillus cereus in cooked rice was examined using plate counting and flow cytometry (FCM). Concurrently, toxigenic potential was measured through recording phosphatidylcholine-specific phospholipase C (PC-PLC) activity. Rice spiked with endospores was incubated at 4 °C, 10 °C or 18 °C for 6 days. Growth was not recorded at 4 °C, whereas >1.0 × 106 CFU g-1 were detected at 10 °C and 18 °C. PC-PLC activity was temperature dependent and was not destroyed by microwaving. FCM population profiling complemented plate count data, providing novel physiological data e.g. on the membrane integrity, redox or intracellular activities of cells over time during low temperature incubation.",

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AB - The effect of storage temperature abuse on the growth and physiology of Bacillus cereus in cooked rice was examined using plate counting and flow cytometry (FCM). Concurrently, toxigenic potential was measured through recording phosphatidylcholine-specific phospholipase C (PC-PLC) activity. Rice spiked with endospores was incubated at 4 °C, 10 °C or 18 °C for 6 days. Growth was not recorded at 4 °C, whereas >1.0 × 106 CFU g-1 were detected at 10 °C and 18 °C. PC-PLC activity was temperature dependent and was not destroyed by microwaving. FCM population profiling complemented plate count data, providing novel physiological data e.g. on the membrane integrity, redox or intracellular activities of cells over time during low temperature incubation.

KW - Bacillus cereus

KW - Flow cytometry

KW - Phosphatidylcholine-specific phospholipase C

KW - Rice

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ER -

The growth, physiology and toxigenic potential of Bacillus cereus in cooked rice during storage temperature abuse

Abstract

Keywords

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