TY - JOUR
T1 - The menaquinol-oxidizing cytochrome bc complex from Thermus thermophilus
T2 - Protein domains and subunits
AU - Mooser, Daniela
AU - Maneg, Oliver
AU - MacMillan, Fraser
AU - Malatesta, Francesco
AU - Soulimane, Tewfik
AU - Ludwig, Bernd
PY - 2006/9
Y1 - 2006/9
N2 - A recently resolved respiratory complex III, isolated from the extreme thermophile Thermus thermophilus, is discussed in terms of cofactor and subunit composition, and with respect to the origin of its protein modules. The four polypeptides, encoded by a single operon, share general homologies to canonical complexes both of the bc and b6f type, but exhibit some unexpected features as well. Evidence for high thermostability of the isolated protein and for its quinol substrate specificity is derived from EPR and kinetic measurements. A functional integration of this complex into an aerobic electron transfer scheme, connecting known dehydrogenase activities to the terminal oxidase branches of Thermus is outlined, as well as the specific principles of redox protein interactions prevailing at high temperature. Findings from this enzyme are linked to present knowledge on other menaquinol oxidizing bc complexes.
AB - A recently resolved respiratory complex III, isolated from the extreme thermophile Thermus thermophilus, is discussed in terms of cofactor and subunit composition, and with respect to the origin of its protein modules. The four polypeptides, encoded by a single operon, share general homologies to canonical complexes both of the bc and b6f type, but exhibit some unexpected features as well. Evidence for high thermostability of the isolated protein and for its quinol substrate specificity is derived from EPR and kinetic measurements. A functional integration of this complex into an aerobic electron transfer scheme, connecting known dehydrogenase activities to the terminal oxidase branches of Thermus is outlined, as well as the specific principles of redox protein interactions prevailing at high temperature. Findings from this enzyme are linked to present knowledge on other menaquinol oxidizing bc complexes.
KW - Complex III
KW - Cytochrome bc
KW - Electron transfer
KW - EPR
KW - Menaquinone
KW - Respiratory chain
KW - Stopped flow kinetic
KW - Thermus thermophilus
UR - http://www.scopus.com/inward/record.url?scp=33748985025&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2006.05.033
DO - 10.1016/j.bbabio.2006.05.033
M3 - Review article
C2 - 16908008
AN - SCOPUS:33748985025
SN - 0005-2728
VL - 1757
SP - 1084
EP - 1095
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 9-10
ER -