The structure of the hyponitrite species in a heme Fe-Cu binuclear center

Constantinos Varotsis, Takehiro Ohta, Teizo Kitagawa, Tewfik Soulimane, Eftychia Pinakoulaki

Research output: Contribution to journalArticlepeer-review

Abstract

NO laughing matter: The enzymatic production of laughing gas (N 2O) from two molecules of NO requires the formation of the N-N bond and the subsequent cleavage of the N-OH bond. Resonance Raman spectroscopic studies have now provided evidence for the formation of the key intermediate, the hyponitrite species (see the optimized molecular structure), in the heme a3 Fe-CuB binuclear center of ba3- oxidoreductase from Thermus thermophilus. (Figure Presented).

Original languageEnglish
Pages (from-to)2210-2214
Number of pages5
JournalAngewandte Chemie - International Edition
Volume46
Issue number13
DOIs
Publication statusPublished - 2007

Keywords

  • Enzymes
  • Metalloenzymes
  • Nitrogen oxides
  • Raman spectroscopy
  • Structure elucidation

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