The use of proteases to remove protein-based residues from solid surfaces

Kevin Turner, Marina Serantoni, Angela Boyce, Gary Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

The efficiency of the commercial proteolytic preparation 'Alcalase' in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by monitoring polypeptide/peptide/amino acid accumulation in the wash and by three-dimensional atomic force microscopy. The results indicate that treatment with enzyme at industrially realistic inclusion levels removes a very significant proportion of protein foulant and that post-cleaning residual enzyme activity on the glass surface can be easily removed or inactivated. Results are discussed in the context of their potential application in the bioprocess sector.

Original languageEnglish
Pages (from-to)3377-3382
Number of pages6
JournalProcess Biochemistry
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 2005

Keywords

  • Alcalase
  • Enzyme-cleaning
  • Fouling
  • Glass
  • Protease

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