Abstract
The efficiency of the commercial proteolytic preparation 'Alcalase' in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by monitoring polypeptide/peptide/amino acid accumulation in the wash and by three-dimensional atomic force microscopy. The results indicate that treatment with enzyme at industrially realistic inclusion levels removes a very significant proportion of protein foulant and that post-cleaning residual enzyme activity on the glass surface can be easily removed or inactivated. Results are discussed in the context of their potential application in the bioprocess sector.
Original language | English |
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Pages (from-to) | 3377-3382 |
Number of pages | 6 |
Journal | Process Biochemistry |
Volume | 40 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 2005 |
Keywords
- Alcalase
- Enzyme-cleaning
- Fouling
- Glass
- Protease