The use of self-interaction chromatography in stable formulation and crystallization of proteins

Kedar S. Deshpande; Tangir Ahamed; Joop H. ter Hoorst; Peter J. Jansens; Luuk A.M. van der Wielen; Marcel Ottens

doi:10.1002/biot.200800226

The use of self-interaction chromatography in stable formulation and crystallization of proteins

Kedar S. Deshpande, Tangir Ahamed, Joop H. ter Hoorst, Peter J. Jansens, Luuk A.M. van der Wielen, Marcel Ottens

Research output: Contribution to journal › Review article › peer-review

Abstract

This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.

Original language	English
Pages (from-to)	1266-1277
Number of pages	12
Journal	Biotechnology Journal
Volume	4
Issue number	9
DOIs	https://doi.org/10.1002/biot.200800226
Publication status	Published - 2009
Externally published	Yes

Keywords

Miniaturization
Predictive protein crystallization
Protein phase behavior
Self-interaction chromatography

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10.1002/biot.200800226

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title = "The use of self-interaction chromatography in stable formulation and crystallization of proteins",

abstract = "This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.",

keywords = "Miniaturization, Predictive protein crystallization, Protein phase behavior, Self-interaction chromatography",

author = "Deshpande, {Kedar S.} and Tangir Ahamed and {ter Hoorst}, {Joop H.} and Jansens, {Peter J.} and {van der Wielen}, {Luuk A.M.} and Marcel Ottens",

year = "2009",

doi = "10.1002/biot.200800226",

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AU - Deshpande, Kedar S.

AU - Ahamed, Tangir

AU - ter Hoorst, Joop H.

AU - Jansens, Peter J.

AU - van der Wielen, Luuk A.M.

AU - Ottens, Marcel

PY - 2009

Y1 - 2009

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AB - This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.

KW - Miniaturization

KW - Predictive protein crystallization

KW - Protein phase behavior

KW - Self-interaction chromatography

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U2 - 10.1002/biot.200800226

DO - 10.1002/biot.200800226

M3 - Review article

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VL - 4

SP - 1266

EP - 1277

JO - Biotechnology Journal

JF - Biotechnology Journal

IS - 9

ER -

The use of self-interaction chromatography in stable formulation and crystallization of proteins

Abstract

Keywords

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