Thermodynamic description of peptide adsorption on mixed-mode resins

Sreekanth Chilamkurthi; David Méndez Sevillano; Leonoor H.G. Albers; Manas Ranjan Sahoo; Peter J.T. Verheijen; Luuk A.M. van der Wielen; Jeroen L.den Hollander; Marcel Ottens

doi:10.1016/j.chroma.2014.03.031

Thermodynamic description of peptide adsorption on mixed-mode resins

Sreekanth Chilamkurthi, David Méndez Sevillano, Leonoor H.G. Albers, Manas Ranjan Sahoo, Peter J.T. Verheijen, Luuk A.M. van der Wielen, Jeroen L.den Hollander, Marcel Ottens

Research output: Contribution to journal › Article › peer-review

Abstract

In this work the adsorption of tri-peptides on a mixed-mode resin was studied using isocratic pulse response experiments. Various salt concentration, temperature and pH combinations were used to measure retention times of several tri-peptides. The experiments were evaluated according to an extension of the stoichiometric displacement model and the steric mass action model of protein-ligand binding. The application of this model in the understanding of mixed mode adsorption process is discussed. A unique set of meaningful thermodynamic parameters was obtained for each resin-peptide-temperature and resin-peptide-pH combination. Finally it was shown that these thermodynamic parameters can be used in defining quantitative relationships within the framework of extra thermodynamic relationships.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	Journal of Chromatography A
Volume	1341
DOIs	https://doi.org/10.1016/j.chroma.2014.03.031
Publication status	Published - 9 May 2014
Externally published	Yes

Keywords

Gibbs free energy
Mixed-mode chromatography
Peptide adsorption
Thermodynamic modelling
Tri-peptides

Access to Document

10.1016/j.chroma.2014.03.031

Cite this

@article{5c9a0d4968014549a8f057c3bada07e3,

title = "Thermodynamic description of peptide adsorption on mixed-mode resins",

abstract = "In this work the adsorption of tri-peptides on a mixed-mode resin was studied using isocratic pulse response experiments. Various salt concentration, temperature and pH combinations were used to measure retention times of several tri-peptides. The experiments were evaluated according to an extension of the stoichiometric displacement model and the steric mass action model of protein-ligand binding. The application of this model in the understanding of mixed mode adsorption process is discussed. A unique set of meaningful thermodynamic parameters was obtained for each resin-peptide-temperature and resin-peptide-pH combination. Finally it was shown that these thermodynamic parameters can be used in defining quantitative relationships within the framework of extra thermodynamic relationships.",

keywords = "Gibbs free energy, Mixed-mode chromatography, Peptide adsorption, Thermodynamic modelling, Tri-peptides",

author = "Sreekanth Chilamkurthi and Sevillano, {David M{\'e}ndez} and Albers, {Leonoor H.G.} and Sahoo, {Manas Ranjan} and Verheijen, {Peter J.T.} and {van der Wielen}, {Luuk A.M.} and Hollander, {Jeroen L.den} and Marcel Ottens",

year = "2014",

month = may,

day = "9",

doi = "10.1016/j.chroma.2014.03.031",

language = "English",

volume = "1341",

pages = "41--49",

journal = "Journal of Chromatography A",

issn = "0021-9673",

}

TY - JOUR

T1 - Thermodynamic description of peptide adsorption on mixed-mode resins

AU - Chilamkurthi, Sreekanth

AU - Sevillano, David Méndez

AU - Albers, Leonoor H.G.

AU - Sahoo, Manas Ranjan

AU - Verheijen, Peter J.T.

AU - van der Wielen, Luuk A.M.

AU - Hollander, Jeroen L.den

AU - Ottens, Marcel

PY - 2014/5/9

Y1 - 2014/5/9

N2 - In this work the adsorption of tri-peptides on a mixed-mode resin was studied using isocratic pulse response experiments. Various salt concentration, temperature and pH combinations were used to measure retention times of several tri-peptides. The experiments were evaluated according to an extension of the stoichiometric displacement model and the steric mass action model of protein-ligand binding. The application of this model in the understanding of mixed mode adsorption process is discussed. A unique set of meaningful thermodynamic parameters was obtained for each resin-peptide-temperature and resin-peptide-pH combination. Finally it was shown that these thermodynamic parameters can be used in defining quantitative relationships within the framework of extra thermodynamic relationships.

AB - In this work the adsorption of tri-peptides on a mixed-mode resin was studied using isocratic pulse response experiments. Various salt concentration, temperature and pH combinations were used to measure retention times of several tri-peptides. The experiments were evaluated according to an extension of the stoichiometric displacement model and the steric mass action model of protein-ligand binding. The application of this model in the understanding of mixed mode adsorption process is discussed. A unique set of meaningful thermodynamic parameters was obtained for each resin-peptide-temperature and resin-peptide-pH combination. Finally it was shown that these thermodynamic parameters can be used in defining quantitative relationships within the framework of extra thermodynamic relationships.

KW - Gibbs free energy

KW - Mixed-mode chromatography

KW - Peptide adsorption

KW - Thermodynamic modelling

KW - Tri-peptides

UR - http://www.scopus.com/inward/record.url?scp=84898538997&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2014.03.031

DO - 10.1016/j.chroma.2014.03.031

M3 - Article

C2 - 24698309

AN - SCOPUS:84898538997

SN - 0021-9673

VL - 1341

SP - 41

EP - 49

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -

Thermodynamic description of peptide adsorption on mixed-mode resins

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this