Three-dimensional crystals of cytochrome-c oxidase from Thermus thermophilus diffracting to 3.8 Å resolution

Tewfik Soulimane; Ulrich Gohlke; Robert Huber; Gerhard Buse

doi:10.1016/0014-5793(95)00623-H

Three-dimensional crystals of cytochrome-c oxidase from Thermus thermophilus diffracting to 3.8 Å resolution

Tewfik Soulimane, Ulrich Gohlke, Robert Huber, Gerhard Buse

Research output: Contribution to journal › Article › peer-review

Abstract

The ba₃-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-β-d-thioglucoside as detergent. The crystals diffract to 3.8 Å, belong to the space group P2 or P2₁ and have unit cell dimensions of a = 80.7 A ̊; b = 116.0 A ̊; c = 156.9 A ̊ and β = 104.4°. The asymmetric unit contains two ba₃-type oxidase molecules.

Original language	English
Pages (from-to)	132-134
Number of pages	3
Journal	FEBS Letters
Volume	368
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00623-H
Publication status	Published - 10 Jul 1995
Externally published	Yes

Keywords

ba-oxidase
cytochrome-c oxidase
Membrane protein crystallization
Thermus thermophilus
X-ray diffraction

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10.1016/0014-5793(95)00623-H

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title = "Three-dimensional crystals of cytochrome-c oxidase from Thermus thermophilus diffracting to 3.8 {\AA} resolution",

abstract = "The ba3-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-β-d-thioglucoside as detergent. The crystals diffract to 3.8 {\AA}, belong to the space group P2 or P21 and have unit cell dimensions of a = 80.7 A ̊; b = 116.0 A ̊; c = 156.9 A ̊ and β = 104.4°. The asymmetric unit contains two ba3-type oxidase molecules.",

keywords = "ba-oxidase, cytochrome-c oxidase, Membrane protein crystallization, Thermus thermophilus, X-ray diffraction",

author = "Tewfik Soulimane and Ulrich Gohlke and Robert Huber and Gerhard Buse",

year = "1995",

month = jul,

day = "10",

doi = "10.1016/0014-5793(95)00623-H",

language = "English",

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pages = "132--134",

journal = "FEBS Letters",

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T1 - Three-dimensional crystals of cytochrome-c oxidase from Thermus thermophilus diffracting to 3.8 Å resolution

AU - Soulimane, Tewfik

AU - Gohlke, Ulrich

AU - Huber, Robert

AU - Buse, Gerhard

PY - 1995/7/10

Y1 - 1995/7/10

N2 - The ba3-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-β-d-thioglucoside as detergent. The crystals diffract to 3.8 Å, belong to the space group P2 or P21 and have unit cell dimensions of a = 80.7 A ̊; b = 116.0 A ̊; c = 156.9 A ̊ and β = 104.4°. The asymmetric unit contains two ba3-type oxidase molecules.

AB - The ba3-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-β-d-thioglucoside as detergent. The crystals diffract to 3.8 Å, belong to the space group P2 or P21 and have unit cell dimensions of a = 80.7 A ̊; b = 116.0 A ̊; c = 156.9 A ̊ and β = 104.4°. The asymmetric unit contains two ba3-type oxidase molecules.

KW - ba-oxidase

KW - cytochrome-c oxidase

KW - Membrane protein crystallization

KW - Thermus thermophilus

KW - X-ray diffraction

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U2 - 10.1016/0014-5793(95)00623-H

DO - 10.1016/0014-5793(95)00623-H

M3 - Article

C2 - 7615066

AN - SCOPUS:0029024956

SN - 0014-5793

VL - 368

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EP - 134

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Three-dimensional crystals of cytochrome-c oxidase from Thermus thermophilus diffracting to 3.8 Å resolution

Abstract

Keywords

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