Tools used to study how protein complexes are assembled in signaling cascades

Susan Dwane, Patrick A. Kiely

Research output: Contribution to journalArticlepeer-review

Abstract

Most proteins do not function on their own but as part of large signaling complexes that are arranged in every living cell in response to specific environmental cues. Proteins interact with each other either constitutively or transiently and do so with different affinity. When identifying the role played by a protein inside a cell, it is essential to define its particular cohort of binding partners so that the researcher can predict what signaling pathways the protein is engaged in. Once identified and confirmed, the information might allow the interaction to be manipulated by pharmacological inhibitors to help fight disease.

Original languageEnglish
Pages (from-to)247-259
Number of pages13
JournalBioengineered
Volume2
Issue number5
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Affinity tagging
  • Cell signaling
  • Co-immunoprecipitation
  • Peptide array technology
  • Protein complexes
  • Protein-protein interactions

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