TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

Zilei Chen; Pedro Carpio Malia; Riko Hatakeyama; Raffaele Nicastro; Zehan Hu; Marie-Pierre Péli-Gulli; Jieqiong Gao; Taki Nishimura; Elja Eskes; Christopher J Stefan; Joris Winderickx; Jörn Dengjel; Claudio De Virgilio; Christian Ungermann

doi:10.1016/j.cub.2020.10.026

TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

Zilei Chen
, Pedro Carpio Malia
, Riko Hatakeyama
, Raffaele Nicastro
, Zehan Hu
, Marie-Pierre Péli-Gulli
, Jieqiong Gao
, Taki Nishimura
, Elja Eskes
, Christopher J Stefan
, Joris Winderickx
, Jörn Dengjel
, Claudio De Virgilio
, Christian Ungermann

Osnabrück University
University of Fribourg
University College London
Functional Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Chen, Malia, Hatakeyama, et al. demonstrate that the yeast Target of Rapamycin Complex 1 (TORC1) phosphorylates the lipid kinase Fab1, a homolog of metazoan PIKfyve. Fab1 phosphorylation shifts both complexes from the vacuole to signaling endosomes and changes TORC1 activity, suggesting a regulatory feedback loop in cellular signaling from these organelles.

Original language	English
Pages (from-to)	297-309.e8
Journal	Current Biology
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/j.cub.2020.10.026
Publication status	Published - 25 Jan 2021
Externally published	Yes

Keywords

Endosomes/metabolism
Enzyme Assays
Feedback, Physiological
Mechanistic Target of Rapamycin Complex 1/metabolism
Phosphorylation/physiology
Phosphotransferases (Alcohol Group Acceptor)/genetics
Recombinant Proteins/genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins/genetics
Signal Transduction
Vacuoles/metabolism

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10.1016/j.cub.2020.10.026

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title = "TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles",

abstract = "Chen, Malia, Hatakeyama, et al. demonstrate that the yeast Target of Rapamycin Complex 1 (TORC1) phosphorylates the lipid kinase Fab1, a homolog of metazoan PIKfyve. Fab1 phosphorylation shifts both complexes from the vacuole to signaling endosomes and changes TORC1 activity, suggesting a regulatory feedback loop in cellular signaling from these organelles.",

keywords = "Endosomes/metabolism, Enzyme Assays, Feedback, Physiological, Mechanistic Target of Rapamycin Complex 1/metabolism, Phosphorylation/physiology, Phosphotransferases (Alcohol Group Acceptor)/genetics, Recombinant Proteins/genetics, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins/genetics, Signal Transduction, Vacuoles/metabolism",

author = "Zilei Chen and Malia, \{Pedro Carpio\} and Riko Hatakeyama and Raffaele Nicastro and Zehan Hu and Marie-Pierre P{\'e}li-Gulli and Jieqiong Gao and Taki Nishimura and Elja Eskes and Stefan, \{Christopher J\} and Joris Winderickx and J{\"o}rn Dengjel and \{De Virgilio\}, Claudio and Christian Ungermann",

year = "2021",

month = jan,

day = "25",

doi = "10.1016/j.cub.2020.10.026",

language = "English",

volume = "31",

pages = "297--309.e8",

journal = "Current Biology",

issn = "0960-9822",

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}

TY - JOUR

T1 - TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

AU - Chen, Zilei

AU - Malia, Pedro Carpio

AU - Hatakeyama, Riko

AU - Nicastro, Raffaele

AU - Hu, Zehan

AU - Péli-Gulli, Marie-Pierre

AU - Gao, Jieqiong

AU - Nishimura, Taki

AU - Eskes, Elja

AU - Stefan, Christopher J

AU - Winderickx, Joris

AU - Dengjel, Jörn

AU - De Virgilio, Claudio

AU - Ungermann, Christian

PY - 2021/1/25

Y1 - 2021/1/25

N2 - Chen, Malia, Hatakeyama, et al. demonstrate that the yeast Target of Rapamycin Complex 1 (TORC1) phosphorylates the lipid kinase Fab1, a homolog of metazoan PIKfyve. Fab1 phosphorylation shifts both complexes from the vacuole to signaling endosomes and changes TORC1 activity, suggesting a regulatory feedback loop in cellular signaling from these organelles.

AB - Chen, Malia, Hatakeyama, et al. demonstrate that the yeast Target of Rapamycin Complex 1 (TORC1) phosphorylates the lipid kinase Fab1, a homolog of metazoan PIKfyve. Fab1 phosphorylation shifts both complexes from the vacuole to signaling endosomes and changes TORC1 activity, suggesting a regulatory feedback loop in cellular signaling from these organelles.

KW - Endosomes/metabolism

KW - Enzyme Assays

KW - Feedback, Physiological

KW - Mechanistic Target of Rapamycin Complex 1/metabolism

KW - Phosphorylation/physiology

KW - Phosphotransferases (Alcohol Group Acceptor)/genetics

KW - Recombinant Proteins/genetics

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins/genetics

KW - Signal Transduction

KW - Vacuoles/metabolism

U2 - 10.1016/j.cub.2020.10.026

DO - 10.1016/j.cub.2020.10.026

M3 - Article

C2 - 33157024

SN - 0960-9822

VL - 31

SP - 297-309.e8

JO - Current Biology

JF - Current Biology

IS - 2

ER -

TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

Abstract

Keywords

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