TY - JOUR
T1 - Toxicological and bioactivity evaluation of blackcurrant press cake, sea buckthorn leaves and bark from Scots pine and Norway spruce extracts under a green integrated approach
AU - Pap, Nora
AU - Reshamwala, Dhanik
AU - Korpinen, Risto
AU - Kilpeläinen, Petri
AU - Fidelis, Marina
AU - Furtado, Marianna M.
AU - Sant'Ana, Anderson S.
AU - Wen, Mingchun
AU - Zhang, Liang
AU - Hellström, Jarkko
AU - Marnilla, Pertti
AU - Mattila, Pirjo
AU - Sarjala, Tytti
AU - Yang, Baoru
AU - Lima, Amanda dos Santos
AU - Azevedo, Luciana
AU - Marjomäki, Varpu
AU - Granato, Daniel
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/7
Y1 - 2021/7
N2 - Aqueous extracts from blackcurrant press cake (BC), Norway spruce bark (NS), Scots pine bark (SP), and sea buckthorn leaves (SB) were obtained using maceration and pressurized hot water and tested for their bioactivities. Maceration provided the extraction of higher dry matter contents, including total phenolics (TPC), anthocyanins, and condensed tannins, which also impacted higher antioxidant activity. NS and SB extracts presented the highest mean values of TPC and antioxidant activity. Individually, NS extract presented high contents of proanthocyanidins, resveratrol, and some phenolic acids. In contrast, SB contained a high concentration of ellagitannins, ellagic acid, and quercetin, explaining the antioxidant activity and antibacterial effects. SP and BC extracts had the lowest TPC and antioxidant activity. However, BC had strong antiviral efficacy, whereas SP can be considered a potential ingredient to inhibit α-amylase. Except for BC, the other extracts decreased reactive oxygen species (ROS) generation in HCT8 and A549 cells. Extracts did not inhibit the production of TNF-alpha in lipopolysaccharide-stimulated THP-1 macrophages but inhibited the ROS generation during the THP-1 cell respiratory burst. The recovery of antioxidant compounds from these by-products is incentivized for high value-added applications.
AB - Aqueous extracts from blackcurrant press cake (BC), Norway spruce bark (NS), Scots pine bark (SP), and sea buckthorn leaves (SB) were obtained using maceration and pressurized hot water and tested for their bioactivities. Maceration provided the extraction of higher dry matter contents, including total phenolics (TPC), anthocyanins, and condensed tannins, which also impacted higher antioxidant activity. NS and SB extracts presented the highest mean values of TPC and antioxidant activity. Individually, NS extract presented high contents of proanthocyanidins, resveratrol, and some phenolic acids. In contrast, SB contained a high concentration of ellagitannins, ellagic acid, and quercetin, explaining the antioxidant activity and antibacterial effects. SP and BC extracts had the lowest TPC and antioxidant activity. However, BC had strong antiviral efficacy, whereas SP can be considered a potential ingredient to inhibit α-amylase. Except for BC, the other extracts decreased reactive oxygen species (ROS) generation in HCT8 and A549 cells. Extracts did not inhibit the production of TNF-alpha in lipopolysaccharide-stimulated THP-1 macrophages but inhibited the ROS generation during the THP-1 cell respiratory burst. The recovery of antioxidant compounds from these by-products is incentivized for high value-added applications.
KW - Bioactive compounds
KW - Circular economy
KW - Extraction technologies
KW - Free radicals
KW - Industrial by-products
KW - Natural resources
UR - http://www.scopus.com/inward/record.url?scp=85106525677&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2021.112284
DO - 10.1016/j.fct.2021.112284
M3 - Article
C2 - 34044082
AN - SCOPUS:85106525677
SN - 0278-6915
VL - 153
SP - 112284
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
M1 - 112284
ER -