Transformation of the coryneform bacterium cellulomonas flavigena with plasmid DNA via electroporation

S. J. Alemohammad; J. Tony Pembroke

doi:10.1007/BF00163290

Transformation of the coryneform bacterium cellulomonas flavigena with plasmid DNA via electroporation

S. J. Alemohammad, J. Tony Pembroke

Retired Staff

University of Limerick

Research output: Contribution to journal › Article › peer-review

Abstract

Using electroporation we have transformed Cellulomonasflavigena with a shuttle vector (pJA85) derived from the E.coli plasmid pUC8 and the Brevibacteriumlactofermentum plasmid pULRS8. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express antibiotic resistance markers originating in Brevibacterium.

Original language	English
Pages (from-to)	147-148
Number of pages	2
Journal	Biotechnology Techniques
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/BF00163290
Publication status	Published - Mar 1990

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title = "Transformation of the coryneform bacterium cellulomonas flavigena with plasmid DNA via electroporation",

abstract = "Using electroporation we have transformed Cellulomonasflavigena with a shuttle vector (pJA85) derived from the E.coli plasmid pUC8 and the Brevibacteriumlactofermentum plasmid pULRS8. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express antibiotic resistance markers originating in Brevibacterium.",

author = "Alemohammad, {S. J.} and Pembroke, {J. Tony}",

year = "1990",

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AU - Alemohammad, S. J.

AU - Pembroke, J. Tony

PY - 1990/3

Y1 - 1990/3

N2 - Using electroporation we have transformed Cellulomonasflavigena with a shuttle vector (pJA85) derived from the E.coli plasmid pUC8 and the Brevibacteriumlactofermentum plasmid pULRS8. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express antibiotic resistance markers originating in Brevibacterium.

AB - Using electroporation we have transformed Cellulomonasflavigena with a shuttle vector (pJA85) derived from the E.coli plasmid pUC8 and the Brevibacteriumlactofermentum plasmid pULRS8. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express antibiotic resistance markers originating in Brevibacterium.

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DO - 10.1007/BF00163290

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AN - SCOPUS:34249954322

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SP - 147

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

Transformation of the coryneform bacterium cellulomonas flavigena with plasmid DNA via electroporation

Abstract

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