Abstract
Surfactant-aided size-exclusion chromatography (SASEC) is applied to the viral clearance of blood proteins, taking BSA as an example. Fixed bed systems as well as simulated moving bed (SMB) systems are examined. SASEC shows a better performance, of this separation in terms of log reduction value (LRV), productivity of BSA, yield on BSA and solvent consumption compared to normal size-exclusion chromatography (SEC) in fixed bed as well as in SMB systems.
Original language | English |
---|---|
Pages (from-to) | 1441-1449 |
Number of pages | 9 |
Journal | AIChE Journal |
Volume | 53 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2007 |
Externally published | Yes |
Keywords
- Protein purification
- Simulated moving bed
- Size-exclusion chromatography
- Surfactants
- Viral clearance