Viral clearance using surfactant-aided size-exclusion chromatography

D. A. Horneman, M. Ottens, L. A.M. Van Der Wielen, J. T.F. Keurentjes

Research output: Contribution to journalArticlepeer-review

Abstract

Surfactant-aided size-exclusion chromatography (SASEC) is applied to the viral clearance of blood proteins, taking BSA as an example. Fixed bed systems as well as simulated moving bed (SMB) systems are examined. SASEC shows a better performance, of this separation in terms of log reduction value (LRV), productivity of BSA, yield on BSA and solvent consumption compared to normal size-exclusion chromatography (SEC) in fixed bed as well as in SMB systems.

Original languageEnglish
Pages (from-to)1441-1449
Number of pages9
JournalAIChE Journal
Volume53
Issue number6
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Keywords

  • Protein purification
  • Simulated moving bed
  • Size-exclusion chromatography
  • Surfactants
  • Viral clearance

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